Stability and Shape of Hepatitis B Virus Capsids In Vacuo

Authors

  • Charlotte Uetrecht,

    1. Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht (The Netherlands), Fax: (+31) 30-251-8219
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  • Cees Versluis,

    1. Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht (The Netherlands), Fax: (+31) 30-251-8219
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  • Norman R. Watts,

    1. Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892 (USA)
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  • Paul T. Wingfield,

    1. Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892 (USA)
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  • Alasdair C. Steven,

    1. Laboratory of Structural Biology Research, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892 (USA)
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  • Albert J. R. Heck Prof. Dr.

    1. Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht (The Netherlands), Fax: (+31) 30-251-8219
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  • This research was supported by the Netherlands Proteomics Centre and the NIAMS Intramural Research Program of the NIH.

Abstract

original image

Viren im Blick: Mithilfe der Ionenmobilitäts-Massenspektrometrie können die Zustände viraler Aggregate nicht nur bezüglich Masse, sondern auch in ihrer Konformation unterschieden werden. Dieser Ansatz führte zur biophysikalischen Charakterisierung des Hepatitis-B-Virus-Kapsids: Von zwei möglichen ikosaedrischen Anordnungen bleibt die sphärische Kapsidstruktur im Vakuum erhalten (siehe Bild).

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