DNA-Coated AFM Cantilevers for the Investigation of Cell Adhesion and the Patterning of Live Cells

Authors

  • Sonny C. Hsiao,

    1. Department of Chemistry, University of California, Berkeley
    2. Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (USA), Fax: (+1) 510-643-3079
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  • Ailey K. Crow,

    1. Biophysics Graduate Group, University of California, Berkeley (USA)
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  • Wilbur A. Lam,

    1. UCB/UCSF Joint Graduate Group in Bioengineering, University of California, Berkeley (USA)
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  • Carolyn R. Bertozzi Prof.,

    1. Department of Chemistry & Molecular and Cell Biology
    2. Howard Hughes Medical Institute, University of California, Berkeley
    3. Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (USA)
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  • Daniel A. Fletcher Prof.,

    1. Biophysics Graduate Group, University of California, Berkeley
    2. UCB/UCSF Joint Graduate Group in Bioengineering, University of California, Berkeley (USA)
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  • Matthew B. Francis Prof.

    1. Department of Chemistry, University of California, Berkeley
    2. Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (USA), Fax: (+1) 510-643-3079
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  • We thank Z. Gartner, R. Chandra, E. Douglas, and X. Chen for technical assistance and helpful discussions, and especially Z. Gartner for DNA preparation. This work was generously supported by Chemical Sciences, Geosciences, and Biosciences Division, Office of Basic Energy Sciences of the US Department of Energy under Contract No. DE-AC03-76SF00098, a National Research Service Award of National Institutes of Health, and a Hammond Research Fellowship of the National Childhood Cancer Foundation and Children's Oncology Group to W.A.L.

Abstract

original image

Fest im Griff: Die Messung der Rezeptor-Adhäsionsstärke erfordert die präzise Manipulation einzelner Zellen auf einer Kontaktoberfläche. Um lebende Zellen an einer beweglichen Sonde zu verankern, wurden AFM-Hebel mit DNA-Sequenzen versehen, die komplementär zu den Strängen auf der Plasmamembran sind (siehe Bild, bp=Basenpaare). Die Stärke der resultierenden Verknüpfungen kann eingestellt werden, indem man die Länge der DNA-Stränge variiert. Auf diese Weise gelingt ein kontrollierter Transport der Zellen.

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