Zuschrift

DNA-Coated AFM Cantilevers for the Investigation of Cell Adhesion and the Patterning of Live Cells†

  1. Sonny C. Hsiao1,2,
  2. Ailey K. Crow3,
  3. Wilbur A. Lam4,
  4. Carolyn R. Bertozzi Prof.7,8,9,
  5. Daniel A. Fletcher Prof.5,6,
  6. Matthew B. Francis Prof.1,2

Article first published online: 16 SEP 2008

DOI: 10.1002/ange.200802525

Issue

Angewandte Chemie

Angewandte Chemie

Volume 120, Issue 44, pages 8601–8605, October 20, 2008

Additional Information

How to Cite

Hsiao, Sonny C., Crow, Ailey K., Lam, Wilbur A., Bertozzi, Carolyn R., Fletcher, Daniel A. and Francis, Matthew B. (2008), DNA-Coated AFM Cantilevers for the Investigation of Cell Adhesion and the Patterning of Live Cells. Angew. Chem., 120: 8601–8605. doi: 10.1002/ange.200802525

Author Information

  1. 1

    Department of Chemistry, University of California, Berkeley

  2. 2

    Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (USA), Fax: (+1) 510-643-3079

  3. 3

    Biophysics Graduate Group, University of California, Berkeley (USA)

  4. 4

    UCB/UCSF Joint Graduate Group in Bioengineering, University of California, Berkeley (USA)

  5. 5

    Biophysics Graduate Group, University of California, Berkeley

  6. 6

    UCB/UCSF Joint Graduate Group in Bioengineering, University of California, Berkeley (USA)

  7. 7

    Department of Chemistry & Molecular and Cell Biology

  8. 8

    Howard Hughes Medical Institute, University of California, Berkeley

  9. 9

    Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (USA)

  1. †

    We thank Z. Gartner, R. Chandra, E. Douglas, and X. Chen for technical assistance and helpful discussions, and especially Z. Gartner for DNA preparation. This work was generously supported by Chemical Sciences, Geosciences, and Biosciences Division, Office of Basic Energy Sciences of the US Department of Energy under Contract No. DE-AC03-76SF00098, a National Research Service Award of National Institutes of Health, and a Hammond Research Fellowship of the National Childhood Cancer Foundation and Children's Oncology Group to W.A.L.

Publication History

  1. Issue published online: 15 OCT 2008
  2. Article first published online: 16 SEP 2008
  3. Manuscript Revised: 7 JUL 2008
  4. Manuscript Received: 30 MAY 2008

Funded by

  • US Department of Energy. Grant Number: DE-AC03-76SF00098
  • National Institutes of Health
  • National Childhood Cancer Foundation and Children's Oncology Group

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article with Supporting Information (HTML) Get PDF (630K)

Keywords:

  • DNA;
  • Rastersondenverfahren;
  • Stoffwechsel-Engineering;
  • Strukturierung;
  • Zelladhäsion
Thumbnail image of graphical abstract

Fest im Griff: Die Messung der Rezeptor-Adhäsionsstärke erfordert die präzise Manipulation einzelner Zellen auf einer Kontaktoberfläche. Um lebende Zellen an einer beweglichen Sonde zu verankern, wurden AFM-Hebel mit DNA-Sequenzen versehen, die komplementär zu den Strängen auf der Plasmamembran sind (siehe Bild, bp=Basenpaare). Die Stärke der resultierenden Verknüpfungen kann eingestellt werden, indem man die Länge der DNA-Stränge variiert. Auf diese Weise gelingt ein kontrollierter Transport der Zellen.

View Full Article with Supporting Information (HTML) Get PDF (630K)