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Engineering A Uranyl-Specific Binding Protein from NikR

Authors


  • This work and use of the Advanced Photon Source, was supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences under contract numbers DE-FG02-07ER15865 (C.H.) and DE-AC02-06CH11357 (M.P.J. and APS use), and a UChicago–Argonne Strategic Collaborative Initiative grant (C.H. and M.P.J.). We thank P. T. Chivers for the nikR encoding plasmid and are grateful for the use of XOR/BESSRC-CAT and the Actinide Facility. NikR is a Ni2+-dependent transcriptional repressor protein.

Abstract

Ein uranylselektives DNA bindendes Protein wurde ausgehend von dem Nickel(II)-responsiven Protein NikR aus E. coli als Templat entwickelt. Das resultierende NikR′-Protein bindet Uranyl (siehe Bild) mit einer Dissoziationskonstante Kd=53 nM und lagert in Gegenwart von Uranyl selektiv DNA an.

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