Charge-Conversional Polyionic Complex Micelles—Efficient Nanocarriers for Protein Delivery into Cytoplasm

Authors

  • Yan Lee Dr.,

    1. Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan), Fax: (+81) 3-5841-7139
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  • Takehiko Ishii Dr.,

    1. Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
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  • Horacio Cabral Dr.,

    1. Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan), Fax: (+81) 3-5841-7139
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  • Hyun Jin Kim,

    1. Department of Materials Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
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  • Ji-Hun Seo,

    1. Department of Materials Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
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  • Nobuhiro Nishiyama Dr.,

    1. Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan), Fax: (+81) 3-5841-7139
    2. Center for Nanobio Integration, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
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  • Hiroki Oshima,

    1. Department of Materials Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
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  • Kensuke Osada Dr.,

    1. Department of Materials Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
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  • Kazunori Kataoka Prof. Dr.

    1. Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan), Fax: (+81) 3-5841-7139
    2. Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
    3. Department of Materials Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
    4. Center for Nanobio Integration, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
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  • This work was supported by a Core Research for Evolutional Science and Technology (CREST) grant from the Japan Science and Technology Agency (JST).

Abstract

Schleuser und ihre Tricks: Polyionische komplexe (PIC) Micellen, die Citaconamid oder cis-Aconitamid enthalten, wurden entwickelt, um Proteine ins Zellplasma zu transportieren. Die erhöhte Ladungsdichte des transportierten Proteins stabilisierte die PIC-Micellen, ohne dass eine Vernetzung nötig war; eine Ladungsänderung in Endosomen führte zur Dissoziation der PIC-Micellen unter effizienter Freisetzung ihres Inhalts (siehe Bild).

original image

Ancillary