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A Facile System for Encoding Unnatural Amino Acids in Mammalian Cells

Authors

  • Peng R. Chen Dr.,

    1. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 Torry Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-9440
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    • These authors contributed equally to this work.

  • Dan Groff,

    1. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 Torry Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-9440
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    • These authors contributed equally to this work.

  • Jiantao Guo Dr.,

    1. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 Torry Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-9440
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  • Weijia Ou Dr.,

    1. Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121 (USA), Fax: (+1) 858-812-1746
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  • Susan Cellitti Dr.,

    1. Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121 (USA), Fax: (+1) 858-812-1746
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  • Bernhard H. Geierstanger Dr.,

    1. Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121 (USA), Fax: (+1) 858-812-1746
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  • Peter G. Schultz Dr.

    1. Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121 (USA), Fax: (+1) 858-812-1746
    2. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 Torry Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-9440
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  • This work was supported by a grant from the U.S. National Institute of Health R01 GM062159 and the Skaggs Institute for Chemical Biology. This is manuscript 19983 of The Scripps Research Institute.

Abstract

Ein Shuttle-System zur Verwendung von in E. coli selektierten Aminoacyl-tRNA-Synthetasen (aaRSs) für den Einbau von nichtnatürlichen Aminosäuren in Säugerzellen wurde entwickelt. Eine in E. coli selektierte Mutante der Pyrrolysyl-tRNA-Synthetase (PylRS) aminoacyliert selektiv eine Nonsense-Suppressor-tRNA mit einem photoaktivierbaren Lysinderivat. Durch Übertragung dieses orthogonalen tRNA-aaRS-Paares in Säugerzellen gelang es, diese nichtnatürliche Aminosäure selektiv in Proteine einzubauen.

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