Non-Hydrolyzable RNA–Peptide Conjugates: A Powerful Advance in the Synthesis of Mimics for 3′-Peptidyl tRNA Termini

Authors

  • Holger Moroder,

    1. Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria), Fax: (+43) 507-512-2892
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  • Jessica Steger,

    1. Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria), Fax: (+43) 507-512-2892
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  • Dagmar Graber,

    1. Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria), Fax: (+43) 507-512-2892
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  • Katja Fauster,

    1. Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria), Fax: (+43) 507-512-2892
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  • Krista Trappl,

    1. Innsbruck Biocenter, Medical University of Innsbruck, Divison of Genomics and RNomics, 6020 Innsbruck (Austria)
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  • Viter Marquez Dr.,

    1. Gene Center, Department for Chemistry and Biochemistry and Center for Integrated Protein Science (CiPS-M), Ludwig-Maximillians-Universität, 81377 Munich (Germany)
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  • Norbert Polacek Priv.-Doz. Dr.,

    1. Innsbruck Biocenter, Medical University of Innsbruck, Divison of Genomics and RNomics, 6020 Innsbruck (Austria)
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  • Daniel N. Wilson Dr.,

    1. Gene Center, Department for Chemistry and Biochemistry and Center for Integrated Protein Science (CiPS-M), Ludwig-Maximillians-Universität, 81377 Munich (Germany)
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  • Ronald Micura Prof. Dr.

    1. Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria), Fax: (+43) 507-512-2892
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  • H. Moroder and J. Steger contributed equally to this work. Funding by the Austrian Science Foundation FWF (P17864 to R.M.; Y315 to N.P.) and the Ministry of Science and Research (GenAU project consortium “non-coding RNAs” P0726-012-012 to R.M. and D1042-011-011 to N.P) and the Deutsche Forschungsgemeinschaft DFG (WI3285/1-1 to D.N.W.) is acknowledged.

Abstract

Die Translation kleiner Peptide am Ribosom kann diesem eine Resistenz gegen Macrolidantibiotika verleihen. Zur Aufklärung dieses und ähnlicher Phänomene sind stabile RNA-Peptid-Konjugate, die Peptidyl-tRNA imitieren, wünschenswert, besonders für die strukturbiologische Untersuchung von Ribosomen. Eine flexible Festphasensynthesestrategie macht diese Konjugate nun in effizienter Weise zugänglich, ohne Beschränkungen bezüglich der RNA- und Peptidsequenz.

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