Photocontrol of Coiled-Coil Proteins in Living Cells

Authors

  • Fuzhong Zhang Dr.,

    1. Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, M5S 3H6 (Canada)
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  • Katharina A. Timm,

    1. Center for Biological Signaling Studies (BIOSS), Freiburg Institute for Advanced Studies (FRIAS), School of Life Sciences (LIFENET), Institute of Biology III, University of Freiburg, 79104 Freiburg (Germany)
    2. Institute for Biochemistry and Biology, University of Potsdam, 14476 Potsdam, Germany
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  • Katja M. Arndt Prof. Dr.,

    1. Center for Biological Signaling Studies (BIOSS), Freiburg Institute for Advanced Studies (FRIAS), School of Life Sciences (LIFENET), Institute of Biology III, University of Freiburg, 79104 Freiburg (Germany)
    2. Institute for Biochemistry and Biology, University of Potsdam, 14476 Potsdam, Germany
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  • G. Andrew Woolley Prof.

    1. Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, M5S 3H6 (Canada)
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  • We thank Dr. Kristian M. Müller for stimulating discussions and Barbara Sauer for technical assistance. This research was supported by the NSERC and CIHR (F.Z. and G.A.W.), by the Emmy-Noether Program of the DFG (Ar 373/1-3; K.M.A.), by the DAAD (K.A.T. and K.M.A.), and by the Excellence Initiative of the German Federal and State Governments (EXC 294; F.Z., K.A.T., and K.M.A.).

Abstract

original image

Licht schaltet die Aktivität des AP-1-Transkriptionsfaktors, eines Doppelwendel(coiled-coil)-Proteins, in lebenden Zellen mithilfe des dominant negativen Peptids XAFosW (rot-gelbes Bandmodell), das mit einer speziellen internen Azobenzolbrücke modifiziert wurde. Im Dunkeln war XAFosW kaum helical und wenig affin für die Jun-Zielproteine (grün), die Bestrahlung mit 365 nm erhöhte aber Helicität und Affinität.

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