Get access

Quantitative CARS Molecular Fingerprinting of Single Living Cells with the Use of the Maximum Entropy Method

Authors

  • Masanari Okuno,

    1. Department of Chemistry, School of Science, The University of Tokyo, Hongo 7-3-1 Bunkyo-ku Tokyo, 113-0033 (Japan), Fax: (+81) 3818-4621
    Search for more papers by this author
  • Prof. Dr. Hideaki Kano,

    1. Department of Chemistry, School of Science, The University of Tokyo, Hongo 7-3-1 Bunkyo-ku Tokyo, 113-0033 (Japan), Fax: (+81) 3818-4621
    Search for more papers by this author
  • Prof. Dr. Philippe Leproux,

    1. Institut de Recherche XLIM, UMR CNRS, No. 6172, 123 avenue Albert Thomas, 87060 Limoges cedex (France)
    Search for more papers by this author
  • Prof. Dr. Vincent Couderc,

    1. Institut de Recherche XLIM, UMR CNRS, No. 6172, 123 avenue Albert Thomas, 87060 Limoges cedex (France)
    Search for more papers by this author
  • Dr. James P. R. Day,

    1. Biosurface Spectroscopy, FOM Institute for Atomic and Molecular Physics, Science Park 104, 1098 XG Amsterdam (The Netherlands)
    Search for more papers by this author
  • Prof. Dr. Mischa Bonn,

    1. Biosurface Spectroscopy, FOM Institute for Atomic and Molecular Physics, Science Park 104, 1098 XG Amsterdam (The Netherlands)
    Search for more papers by this author
  • Prof. Dr. Hiro-o Hamaguchi

    1. Department of Chemistry, School of Science, The University of Tokyo, Hongo 7-3-1 Bunkyo-ku Tokyo, 113-0033 (Japan), Fax: (+81) 3818-4621
    2. Institute of Molecular Science, National Chiao Tung University, 1001 Ta Hsueh Road, Hsinchu 300 (Taiwan)
    Search for more papers by this author

  • This work was supported by the SENTAN project (Program-S) of the Japan Science and Technology Agency (JST). H. Kano gratefully acknowledges financial support from the Precursory Research for Embryonic Science and Technology (PRESTO) program of JST. The authors thank C. Onogi for providing the spontaneous Raman spectrum of yeast mitochondria, Leukos and Horus Laser companies for technical support, and Dr. F. Omura and H. Yomo (Suntory Co., Ltd.) for providing us with the yeast sample. We gratefully acknowledge J. Ukon (HORIBA, Ltd.) for assisting in the collaboration between the Japanese and French groups.

Abstract

original image

Der Prozess des Zelltods wurde in Echtzeit auf subzellulärer Ebene mithilfe von kohärenter Anti-Stokes-Raman-Mikrospektroskopie verfolgt. Diese Weiterentwicklung der Anti-Stokes-Raman-Streuungs(CARS)-Mikroskopie zeigt Veränderungen des chemischen Kontrastes beim Zelltod in deutlicher Auflösung (siehe Bild).

Get access to the full text of this article

Ancillary