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A Significant Improvement of the Efficacy of Radical Oxidant Probes by the Kinetic Isotope Effect

Authors

  • Kousik Kundu,

    1. The Wallace H. Coulter Department of Biomedical Engineering and Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332 (USA), Fax: (+1) 404-894-4243
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  • Sarah F. Knight,

    1. Cardiology Division, Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322 (USA)
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  • Seungjun Lee,

    1. The Wallace H. Coulter Department of Biomedical Engineering and Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332 (USA), Fax: (+1) 404-894-4243
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  • W. Robert Taylor Prof.,

    1. The Wallace H. Coulter Department of Biomedical Engineering and Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332 (USA), Fax: (+1) 404-894-4243
    2. Cardiology Division, Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322 (USA)
    3. Cardiology Division, Atlanta VA Medical Center, Decatur, GA 30032 (USA)
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  • Niren Murthy Prof.

    1. The Wallace H. Coulter Department of Biomedical Engineering and Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332 (USA), Fax: (+1) 404-894-4243
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  • This work was supported by the Georgia Tech/Emory Center for the Engineering of Living Tissues (funded by NSF-EEC-9731643) (N.M.), NSF-BES-0546962 Career Award (N.M.), NIH UO1 HL80711-01 (N.M.), NIH R21 EB006418 (N.M.) NIH RO1 HL096796-01 (N.M.), NIH RO1 HL090584 (W.R.T.) and J&J/GT Health Care Innovation Seed Grant Proposal (N.M.).

Abstract

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Der Grad der Oxidation von Dihydroethidium- und Hydrocyanin-Sonden für radikalische Oxidationsmittel durch Luft kann durch Deuterierung selektiv reduziert werden (siehe Bild). Die Geschwindigkeit der Reaktion der deuterierten Verbindungen mit Superoxid-Radikalen wurde aufgrund mechanistischer Unterschiede deutlich weniger verringert. Die deuterierten Sonden sind in vitro, in der Zellkultur und in vivo effizienter als die nichtdeuterierten.

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