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A Fluorescent Rosamine Compound Selectively Stains Pluripotent Stem Cells

Authors

  • Dr. Chang-Nim Im,

    1. Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543 (Republic of Singapore), Fax: (+65) 6779-1691 http://ytchang.science.nus.edu.sg
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    • These authors contributed equally to this work.

  • Dr. Nam-Young Kang,

    1. Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, # 02-02 Helios, Singapore 138667 (Republic of Singapore)
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    • These authors contributed equally to this work.

  • Dr. Hyung-Ho Ha,

    1. Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543 (Republic of Singapore), Fax: (+65) 6779-1691 http://ytchang.science.nus.edu.sg
    2. NUS MedChem Program of Life Sciences Institute, National University of Singapore, Singapore 117543 (Republic of Singapore)
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  • Dr. Xuezhi Bi,

    1. Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, # 02-02 Helios, Singapore 138667 (Republic of Singapore)
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  • Dr. Jae Jung Lee,

    1. Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, # 02-02 Helios, Singapore 138667 (Republic of Singapore)
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  • Dr. Sung-Jin Park,

    1. Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, # 02-02 Helios, Singapore 138667 (Republic of Singapore)
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  • Dr. Sang Yeon Lee,

    1. Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543 (Republic of Singapore), Fax: (+65) 6779-1691 http://ytchang.science.nus.edu.sg
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  • Dr. Marc Vendrell,

    1. Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, # 02-02 Helios, Singapore 138667 (Republic of Singapore)
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  • Dr. Yun Kyung Kim,

    1. Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543 (Republic of Singapore), Fax: (+65) 6779-1691 http://ytchang.science.nus.edu.sg
    2. Department of Chemistry, New York University, New York, NY 10003 (USA)
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  • Dr. Jun-Seok Lee,

    1. Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543 (Republic of Singapore), Fax: (+65) 6779-1691 http://ytchang.science.nus.edu.sg
    2. Department of Chemistry, New York University, New York, NY 10003 (USA)
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  • Dr. Jun Li,

    1. Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, # 02-02 Helios, Singapore 138667 (Republic of Singapore)
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  • Dr. Young-Hoon Ahn,

    1. Department of Chemistry, New York University, New York, NY 10003 (USA)
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  • Dr. Bo Feng,

    1. Gene Regulation Laboratory, Genome Institute of Singapore, Agency for Science, Technology and Research (A*STAR), Singapore 138672 (Republic of Singapore)
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  • Dr. Huck-Hui Ng,

    1. Gene Regulation Laboratory, Genome Institute of Singapore, Agency for Science, Technology and Research (A*STAR), Singapore 138672 (Republic of Singapore)
    2. Department of Biological Sciences, National University of Singapore, Singapore 117543 (Republic of Singapore)
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  • Dr. Seong-Wook Yun,

    1. Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, # 02-02 Helios, Singapore 138667 (Republic of Singapore)
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  • Prof. Dr. Young-Tae Chang

    1. Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543 (Republic of Singapore), Fax: (+65) 6779-1691 http://ytchang.science.nus.edu.sg
    2. Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, # 02-02 Helios, Singapore 138667 (Republic of Singapore)
    3. NUS MedChem Program of Life Sciences Institute, National University of Singapore, Singapore 117543 (Republic of Singapore)
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  • We thank Sai Kiang Lim (Institute of Medical Biology, Singapore) for providing mESC and MEF and kind consultation. We also thank Siti Hajar, Chew Yan Tuang, Yee Ling Tan, Pei Fen Tay, and Si Qiang Yang for excellent technical support in cell culture and screening, and Clement Khaw (SBIC-Nikon Imaging Centre) for microscopy. This work was supported by a Young Investigator Award (R-143-000-353-101) granted to Y.-T.C. from the National University of Singapore and intramural funding from the A*STAR Biomedical Research Council. J.-S.L. was supported by a Korea Research Foundation Grant funded by the Korean government (MOEHRD, Basic Research Promotion Fund: KRF-2005-C00088).

Abstract

original image

Ein gutes Image: Die fluoreszierende Verbindung CDy1 färbt selektiv embryonische Stammzellen an (siehe Schema). CDy1 wurde zur Identifizierung von Fibroblasten verwendet, die eine Umprogrammierung zu pluripotenten, GFP exprimierenden Stammzellen eingehen.

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