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Amplified Aptamer-Based Assay through Catalytic Recycling of the Analyte

Authors

  • Chun-Hua Lu,

    1. The Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002 (China), Fax: (+86) 591-2286-6135
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  • Juan Li,

    1. The Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002 (China), Fax: (+86) 591-2286-6135
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  • Mei-Hua Lin,

    1. The Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002 (China), Fax: (+86) 591-2286-6135
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  • Yi-Wei Wang,

    1. The Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002 (China), Fax: (+86) 591-2286-6135
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  • Prof. Huang-Hao Yang,

    1. The Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002 (China), Fax: (+86) 591-2286-6135
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  • Prof. Xi Chen,

    1. The Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002 (China), Fax: (+86) 591-2286-6135
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  • Prof. Guo-Nan Chen

    1. The Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002 (China), Fax: (+86) 591-2286-6135
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  • This research was supported by the National Natural Science Foundation of China (No. 20735002, No. 20975023), the National Basic Research Program of China (No. 2010CB732403), and the “Program for New Century Excellent Talents in University” of China.

Abstract

original image

In einem einfachen und hoch empfindlichen Test auf Aptamerbasis, bei dem die DNA an Nanomaterialien vor der Spaltung durch Nuclease geschützt wird, verlässt das Aptamer auf Kontakt mit dem Analyt hin das Nanomaterial. Die Nuclease spaltet anschließend das freie Aptamer und setzt den Analyt wieder frei. Somit kann der Analyt einen weiteren Zyklus auslösen, was in einer deutlichen Signalverstärkung resultiert (siehe Schema).

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