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cat-ELCCA: A Robust Method To Monitor the Fatty Acid Acyltransferase Activity of Ghrelin O-Acyltransferase (GOAT)

Authors

  • Dr. Amanda L. Garner,

    1. Departments of Chemistry and Immunology, The Skaggs Institute for Chemical Biology and The Worm Institute for Research and Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-2595
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  • Prof. Kim D. Janda

    Corresponding author
    1. Departments of Chemistry and Immunology, The Skaggs Institute for Chemical Biology and The Worm Institute for Research and Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-2595
    • Departments of Chemistry and Immunology, The Skaggs Institute for Chemical Biology and The Worm Institute for Research and Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 (USA), Fax: (+1) 858-784-2595
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  • This work was supported by the Skaggs Institute for Chemical Biology (K.D.J.) and by the NIH (postdoctoral fellowship F32-DK083179 to A.L.G.). We are grateful to Damian Ekiert for assistance with baculovirus expression, Prof. M. G. Finn for helpful discussions, and Vu Hong for donation of THPTA.

Abstract

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Assays mit katalytischer Signalverstärkung sind überlegene Systeme für hoch empfindliche Nachweisverfahren. Ein neuartiger Enzym-Assay namens cat-ELCCA (catalytic assay using enzyme-linked click-chemistry) wird beschrieben, bei dem ein enzymgebundenes Azid die katalytische Fluoreszenzverstärkung bewirkt. Diese Technik kam beim ersten Hochdurchsatz-Screennig auf die kürzlich entdeckte Ghrelin-O-Acyltransferase (GOAT) zur Anwendung.

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