Superresolution Optical Fluctuation Imaging with Organic Dyes

Authors

  • Dr. Thomas Dertinger,

    Corresponding author
    1. Department of Chemistry/Biochemistry, California NanoSystems Institute, University of California Los Angeles, 607 Charles E. Young Drive E, Los Angeles, CA, 90095 (USA), Fax: (+1) 310 267 4672
    • Department of Chemistry/Biochemistry, California NanoSystems Institute, University of California Los Angeles, 607 Charles E. Young Drive E, Los Angeles, CA, 90095 (USA), Fax: (+1) 310 267 4672
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  • Dr. Mike Heilemann,

    Corresponding author
    1. Department of Physics, Applied Laser Physics, Bielefeld University, Universitätsstrasse 25, 33615 Bielefeld (Germany)
    • Department of Physics, Applied Laser Physics, Bielefeld University, Universitätsstrasse 25, 33615 Bielefeld (Germany)
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  • Robert Vogel,

    1. Department of Chemistry/Biochemistry, California NanoSystems Institute, University of California Los Angeles, 607 Charles E. Young Drive E, Los Angeles, CA, 90095 (USA), Fax: (+1) 310 267 4672
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  • Prof. Markus Sauer,

    1. Department of Biotechnology and Biophysics, Julius-Maximilians-Universität Würzburg, Am Hubland, 97074 Würzburg (Germany)
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  • Prof. Shimon Weiss

    Corresponding author
    1. Department of Chemistry/Biochemistry, California NanoSystems Institute, University of California Los Angeles, 607 Charles E. Young Drive E, Los Angeles, CA, 90095 (USA), Fax: (+1) 310 267 4672
    • Department of Chemistry/Biochemistry, California NanoSystems Institute, University of California Los Angeles, 607 Charles E. Young Drive E, Los Angeles, CA, 90095 (USA), Fax: (+1) 310 267 4672
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  • This work was supported by the Biophotonics and Systems Biology Initiative (FORSYS) of the German Ministry of Research and Education (BMBF; grants 13N9234 and 0135262). T.D. was supported by the German Science Foundation (DFG, fellowship DE 1591/1-1) and by a National Institutes of Health grant (5R01EB000312).

Abstract

original image

SOFI steht für „Superresolution Optical Fluctuation Imaging“, die jüngste einer Reihe sehr hoch auflösender Weitfeldmikroskopie-Techniken. Das Verfahren benötigt nur wenig Aufnahmezeit und lässt sich auf Proben anwenden, die mit herkömmlichen organischen Farbstoffen markiert sind.

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