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Identification of Drug Targets In Vitro and in Living Cells by Soluble-Nanopolymer-Based Proteomics

Authors

  • Lianghai Hu,

    1. Department of Biochemistry, Purdue University, West Lafayette, IN 47907 (USA)
    2. College of Life Science, Jilin University, Changchun 130012 (PR China)
    3. Center for Cancer Research, Purdue University
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  • Anton Iliuk,

    1. Department of Biochemistry, Purdue University, West Lafayette, IN 47907 (USA)
    2. Center for Cancer Research, Purdue University
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  • Jacob Galan,

    1. Department of Biochemistry, Purdue University, West Lafayette, IN 47907 (USA)
    2. Center for Cancer Research, Purdue University
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  • Michael Hans,

    1. Department of Biochemistry, Purdue University, West Lafayette, IN 47907 (USA)
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  • W. Andy Tao

    Corresponding author
    1. Department of Biochemistry, Purdue University, West Lafayette, IN 47907 (USA)
    2. Center for Cancer Research, Purdue University
    3. Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University
    4. Department of Chemistry, Purdue University
    • Department of Biochemistry, Purdue University, West Lafayette, IN 47907 (USA)
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  • This project was funded in part by a NSF CAREER award, the 3M general fund, and the National Institutes of Health (grant 5R21RR025802). We are particularly grateful for a generous NIH high-end-instrumentation grant (S10RR025044) for the purchase of an LTQ Orbitrap Velos mass spectrometer.

Abstract

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Wirkstofftragende Dendrimere ermöglichten in Kombination mit massenspektrometrischer Analyse, Wirkstoffziele in vitro und in lebenden Zellen zu identifizieren. Dabei wird das wirkstofftragende Nanopolymer mit Zellen inkubiert, um einen effizienten Transport in die Zellen sicherzustellen (siehe Bild), und die Zellen werden anschließend lysiert. Wirkstoffgebundene Proteine werden auf einem festen Träger isoliert und massenspektrometrisch identifiziert.

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