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Use of Perfluorocarboxylic Acids To Trick Cytochrome P450BM3 into Initiating the Hydroxylation of Gaseous Alkanes

Authors

  • Dr. Norifumi Kawakami,

    1. Research Center for Materials Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602 (Japan), Fax: (+81) 52-789-2953
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  • Dr. Osami Shoji,

    1. Department of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602 (Japan)
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  • Prof. Dr. Yoshihito Watanabe

    Corresponding author
    1. Research Center for Materials Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602 (Japan), Fax: (+81) 52-789-2953
    • Research Center for Materials Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602 (Japan), Fax: (+81) 52-789-2953
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  • Two plasmids encoding the full length of P450BM3 and the heme domain of P450BM3 were kindly supplied by Prof. S. G. Sligar, University of Illinois (USA). This research was supported by a Grant-in-Aid for Scientific Research (S) to Y.W. (19105044) from the Ministry of Education, Culture, Sports, Science, and Technology (Japan) and a Grant-in-Aid for Young Scientists (A) to O.S. (21685018).

Abstract

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Sie kann's doch! Lange wurde geglaubt, der Wildtyp der Fettsäure-Hydroxylase P450BM3 sei nicht befähigt, gasförmige Alkane zu oxidieren. Die einfache Zugabe einer Perfluorcarbonsäure als Blindsubstrat zur Initiierung des P450BM3-Katalysezyklus ermöglichte jedoch die effiziente Hydroxylierung von Butan und Propan (siehe Bild).

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