Zuschrift

Tracking N-Acetyllactosamine on Cell-Surface Glycans In Vivo†

  1. Tianqing Zheng1,
  2. Dr. Hao Jiang1,
  3. Dr. Marilyn Gros2,
  4. Dr. David Soriano del Amo1,
  5. Subha Sundaram3,
  6. Prof. Grégoire Lauvau2,
  7. Prof. Florence Marlow4,
  8. Dr. Yi Liu5,
  9. Prof. Pamela Stanley3,
  10. Prof. Peng Wu1,*

Article first published online: 29 MAR 2011

DOI: 10.1002/ange.201100265

Issue

Angewandte Chemie

Angewandte Chemie

Volume 123, Issue 18, pages 4199–4204, April 26, 2011

Additional Information

How to Cite

Zheng, T., Jiang, H., Gros, M., Soriano del Amo, D., Sundaram, S., Lauvau, G., Marlow, F., Liu, Y., Stanley, P. and Wu, P. (2011), Tracking N-Acetyllactosamine on Cell-Surface Glycans In Vivo. Angew. Chem., 123: 4199–4204. doi: 10.1002/ange.201100265

Author Information

  1. 1

    Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022

  2. 2

    Department of Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University (USA)

  3. 3

    Department of Cell Biology, Albert Einstein College of Medicine of Yeshiva University (USA)

  4. 4

    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine of Yeshiva University (USA)

  5. 5

    The Molecular Foundry, Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley (USA)

*Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022

  1. †

    This work was supported by the National Institutes of Health (R01GM093282 to P.W. and R01CA036434 to P.S.) and the Mizutani Foundation for Glycoscience (to P.W.). Y.L. was supported by the Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under contract No. DE-AC02-05 CH11231. We thank Prof. Carolyn Bertozzi for providing DIFO-647. Two disaccharides used in the kinetic assay were provided by The Consortium for Functional Glycomics (funded by NIGMS-GM62116).

Publication History

  1. Issue published online: 20 APR 2011
  2. Article first published online: 29 MAR 2011
  3. Manuscript Received: 12 JAN 2011

Funded by

  • National Institutes of Health. Grant Numbers: R01GM093282, R01CA036434
  • Mizutani Foundation for Glycoscience
  • U.S. Department of Energy. Grant Numbers: DE-AC02-05 CH11231, NIGMS-GM62116

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Keywords:

  • Klick-Chemie;
  • Enzymkatalyse;
  • Glycokonjugate;
  • Glycosylierungen;
  • Transferasen
Thumbnail image of graphical abstract

Viele Säugetier-Glycane, die mit Signalrezeptoren assoziiert sind, enthalten N-Acetyllactosamin an der letzten oder vorletzten Position. Eine hochspezifische Methode zur Markierung dieses Disaccharids auf Zelloberflächen-Glycoproteinen lebender, kultivierter Zellen und Zebrafischembryos nutzt einen zweistufigen chemoenzymatischen Ansatz aus In-situ-Fucosylierung und bioorthogonaler Klickreaktion (siehe Schema; α(1,3)FucT=α(1,3)-Fucosyltransferase).

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