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Biosynthesis of Himastatin: Assembly Line and Characterization of Three Cytochrome P450 Enzymes Involved in the Post-tailoring Oxidative Steps

Authors

  • Dr. Junying Ma,

    1. CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301 (China)
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  • Dr. Zhongwen Wang,

    1. CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301 (China)
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  • Dr. Hongbo Huang,

    1. CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301 (China)
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  • Minghe Luo,

    1. CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301 (China)
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  • Dianguang Zuo,

    1. CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301 (China)
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  • Dr. Bo Wang,

    1. CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301 (China)
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  • Aijun Sun,

    1. CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301 (China)
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  • Prof. Dr. Yi-Qiang Cheng,

    1. Department of Biological Sciences, University of Wisconsin–Milwaukee, P.O. Box 413, Milwaukee, WI 53201 (USA)
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  • Prof. Dr. Changsheng Zhang,

    1. CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301 (China)
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  • Prof. Dr. Jianhua Ju

    Corresponding author
    1. CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301 (China)
    • CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301 (China)
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  • This work was financially supported by grants from the NSFC (20872152), MOST (2010CB833805), and the Knowledge Innovation Programs of the CAS (KZCX2-YW-JC202, KSCX2-YW-G-065, LYQY200805, and KZCX2-EW-G-12). J.J. is a scholar of the “100 Talents Program” of the Chinese Academy of Sciences (08L111001).

Abstract

original image

Der Biosyntheseweg von Himastatin aus Streptomyces himastatinicus wurde durch Genomanalyse, die Erzeugung von Gendeletionsmutanten und Komplementierungsexperimente aufgeklärt. Drei Cytochrome P450, die neuartige postmodifizierende Oxidationen katalysieren (siehe Bild), werden charakterisiert.

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