Site-Specific pKa Determination of Selenocysteine Residues in Selenovasopressin by Using 77Se NMR Spectroscopy

Authors

  • Dr. Mehdi Mobli,

    Corresponding author
    1. Department of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St Lucia QLD 4072 (Australia)
    • Department of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St Lucia QLD 4072 (Australia)
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  • David Morgenstern,

    1. Department of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St Lucia QLD 4072 (Australia)
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  • Prof. Glenn F. King,

    1. Department of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St Lucia QLD 4072 (Australia)
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  • Prof. Paul F. Alewood,

    1. Department of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St Lucia QLD 4072 (Australia)
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  • Dr. Markus Muttenthaler

    Corresponding author
    1. Department of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St Lucia QLD 4072 (Australia)
    • Department of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St Lucia QLD 4072 (Australia)
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  • This work was supported by Discovery Grant DP1095728 from the Australian Research Council and the Internationally Outgoing Marie Curie Fellowship. We thank NMR facility manager P. Harvey at the IMB for technical support.

Abstract

original image

Selenocystein (Sec), die 21. Aminosäure, kommt in Proteinen aller Lebensformen vor, und die höhere Säurestärke von Selenol im Vergleich zu Thiolen verleiht ihr besondere chemische Eigenschaften. 77Se-NMR-Spektroskopie lieferte den pKa-Wert von Sec-Resten in einem Protein in atomarer Auflösung. Diese Information kann genutzt werden, um chemische Reaktionen vorherzusagen.

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