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A Glycopeptide Dendrimer Inhibitor of the Galactose-Specific Lectin LecA and of Pseudomonas aeruginosa Biofilms

Authors

  • Dr. Rameshwar U. Kadam,

    1. Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne (Switzerland)
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  • Myriam Bergmann,

    1. Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne (Switzerland)
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  • Dr. Matthew Hurley,

    1. School of Molecular Medical Sciences, University of Nottingham, Nottingham NG7 2UH (UK)
    2. School of Clinical Sciences, University of Nottingham, Nottingham NG7 2UH (UK)
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  • Dr. Divita Garg,

    1. Institute of Structural Biology, Helmholtz Zentrum München and Center for Integrated Protein Science Munich, Departement Chemie, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany)
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  • Dr. Martina Cacciarini,

    1. Dipartimento di Chimica, Polo Scientifico e Tecnologico, Universita' degli Studi di Firenze, Via della Lastruccia 3, 13, 50019 Sesto Fiorentino—Firenze (Italy)
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  • Dr. Magdalena A. Swiderska,

    1. Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne (Switzerland)
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  • Prof. Cristina Nativi,

    1. Dipartimento di Chimica, Polo Scientifico e Tecnologico, Universita' degli Studi di Firenze, Via della Lastruccia 3, 13, 50019 Sesto Fiorentino—Firenze (Italy)
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  • Prof. Dr. Michael Sattler,

    1. Institute of Structural Biology, Helmholtz Zentrum München and Center for Integrated Protein Science Munich, Departement Chemie, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany)
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  • Prof. Alan R. Smyth,

    1. School of Clinical Sciences, University of Nottingham, Nottingham NG7 2UH (UK)
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  • Prof. Paul Williams,

    1. School of Molecular Medical Sciences, University of Nottingham, Nottingham NG7 2UH (UK)
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  • Prof. Miguel Cámara,

    1. School of Molecular Medical Sciences, University of Nottingham, Nottingham NG7 2UH (UK)
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  • Priv.-Doz. Dr. Achim Stocker,

    1. Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne (Switzerland)
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  • Priv.-Doz. Dr. Tamis Darbre,

    1. Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne (Switzerland)
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  • Prof. Dr. Jean-Louis Reymond

    Corresponding author
    1. Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne (Switzerland)
    • Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne (Switzerland)
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  • This work was supported by the University of Berne, the Swiss NSF, the Swiss OFES, and the COST action D34. M.A.S. is supported by the Novartis Foundation. M.H. is supported by the Wellcome Trust. We thank Dr. A. Imberty for a gift of the LecA expression vector and the staff at SLS beamline PX-II/III for support during data collection.

Abstract

original image

Inhibierende Faktoren: Die Bildung von Biofilmen kann mithilfe eines Phenylgalactosyl-Peptid-Dendrimers (siehe Bild), das an das galactosespezifische Lectin LecA von P. aeruginosa bindet, unterdrückt werden. Die Multivalenz des Liganden ist entscheidend für die Inhibierung der Biofilmbildung, wobei der Linker zwischen Peptiddendrimer und Galactose zusätzliche Kontakte mit dem Lectin ermöglicht und somit ebenfalls die Wechselwirkung beeinflusst.

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