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Fluorescence Detection of Intron Lariat RNA with Reduction-Triggered Fluorescent Probes

Authors

  • Dr. Kazuhiro Furukawa,

    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198 (Japan)
    2. Department of Life Science and Medical Bio-Science, Waseda University, 2-2, Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480 (Japan)
    3. Department of Molecular, Cellular, and Developmental Biology, Yale University.
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  • Prof. Dr. Hiroshi Abe,

    Corresponding author
    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198 (Japan)
    2. PRESTO, Japan Science and Technology Agency, 4-1-8, Honcho, Kawaguchi, Saitama 332-0012, (Japan)
    • Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198 (Japan)
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  • Yasutsugu Tamura,

    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198 (Japan)
    2. Department of Life Science and Medical Bio-Science, Waseda University, 2-2, Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480 (Japan)
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  • Dr. Rei Yoshimoto,

    1. Chemical Genetics Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198 (Japan)
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  • Prof. Dr. Minoru Yoshida,

    1. Chemical Genetics Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198 (Japan)
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  • Prof. Dr. Satoshi Tsuneda,

    1. Department of Life Science and Medical Bio-Science, Waseda University, 2-2, Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480 (Japan)
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  • Prof. Dr. Yoshihiro Ito

    Corresponding author
    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198 (Japan)
    • Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198 (Japan)
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  • K.F., H.A., and Y.I. were financially supported by JSPS, MEXT, and NEDO and MEXT, respectively. We thank the BSI Research Resources Center for the mass spectrum analysis and Dr. A. Roth for helpful comments on the manuscript.

Abstract

original image

Fluoreszenz durch Nähe: Reduktionsinduzierte Fluoreszenzsonden, die ein Fluorescein-Derivat oder Triphenylphosphan tragen, binden sequenzspezifisch am Verzweigungspunkt von Lariat-RNA. Die chemische Reaktion der beiden Substrate führt zur Bildung von nativem Fluorescein und zur Emission eines Fluoreszenzsignals (siehe Bild). Prälariat- und Lariat-Strukturen können mit dieser Methode unterschieden werden.

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