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Segmental Isotopic Labeling of Ubiquitin Chains To Unravel Monomer-Specific Molecular Behavior

Authors

  • Dr. Carlos A. Castañeda,

    1. Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, College Park, MD 20742 (USA)
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  • Liat Spasser,

    1. Department of Chemistry and National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer Sheva, 84105 (Israel)
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  • Dr. Sudhir N. Bavikar,

    1. Department of Chemistry and National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer Sheva, 84105 (Israel)
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  • Prof. Ashraf Brik,

    Corresponding author
    1. Department of Chemistry and National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer Sheva, 84105 (Israel)
    • Department of Chemistry and National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer Sheva, 84105 (Israel)
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  • Prof. David Fushman

    Corresponding author
    1. Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, College Park, MD 20742 (USA)
    • Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, College Park, MD 20742 (USA)
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  • Supported by NIH grant GM065334 to D.F., NSF Postdoctoral Fellowship DBI-0905967 to C.A.C., and by the Edmond J. Safra Foundation and Israel Science Foundation (A.B.).

Abstract

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Nimm zwei: Durch Kombination von chemischer Synthese eines Ubiquitinmonomers mit rekombinanter Expression seines 15N-Gegenstücks gelang die Markierung von Ubiquitinen in nichtenzymatisch aufgebauten Diubiquitinketten, was monomerspezifische Studien in Lösung ermöglichte. So konnten für Lys33-verknüpftes Diubiquitin erstmals die Struktur, Dynamik, Ligandenbindung und Wechselwirkungen innerhalb der Kette charakterisiert werden.

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