Zuschrift

Solution NMR Structure of Proteorhodopsin†

  1. Sina Reckel1,‡,
  2. Daniel Gottstein1,‡,
  3. Jochen Stehle2,
  4. Dr. Frank Löhr1,
  5. Dr. Mirka-Kristin Verhoefen3,
  6. Dr. Mitsuhiro Takeda4,
  7. Robert Silvers2,
  8. Prof. Dr. Masatsune Kainosho4,6,
  9. Prof. Dr. Clemens Glaubitz1,
  10. Prof. Dr. Josef Wachtveitl3,
  11. Dr. Frank Bernhard1,
  12. Prof. Dr. Harald Schwalbe2,
  13. Prof. Dr. Peter Güntert1,5,6,
  14. Prof. Dr. Volker Dötsch1,*

Article first published online: 27 OCT 2011

DOI: 10.1002/ange.201105648

Issue

Angewandte Chemie

Angewandte Chemie

Volume 123, Issue 50, pages 12148–12152, December 9, 2011

Additional Information

How to Cite

Reckel, S., Gottstein, D., Stehle, J., Löhr, F., Verhoefen, M.-K., Takeda, M., Silvers, R., Kainosho, M., Glaubitz, C., Wachtveitl, J., Bernhard, F., Schwalbe, H., Güntert, P. and Dötsch, V. (2011), Solution NMR Structure of Proteorhodopsin. Angew. Chem., 123: 12148–12152. doi: 10.1002/ange.201105648

Author Information

  1. 1

    Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)

  2. 2

    Institute for Organic Chemistry and Chemical Biology and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 7–9, 60438 Frankfurt (Germany)

  3. 3

    Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Max-von-Laue Str. 7, 60438 Frankfurt (Germany)

  4. 4

    Structural Biology Research Center, Nagoya University, Furo-cho, Chikusa-ku, 464-8601 (Japan)

  5. 5

    Frankfurt Institute for Advanced Studies, Goethe University Frankfurt, Ruth-Moufang-Str.1, 60438 Frankfurt am Main (Germany)

  6. 6

    Center for Priority Areas, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397 (Japan)

  1. ‡

    These authors contributed equally to this work.

*Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)

  1. †

    This work was supported by the Deutsche Forschungsgemeinschaft (grant number DO545/7-1 and SFB 807), the European Drug Initiative on Channels and Transporters (EDICT) contract number HEALTH-F4-2007-201924, the NIH (grant number U54 GM094608), the Center for Biomolecular Magnetic Resonance (BMRZ) and the Cluster of Excellence Frankfurt (Macromolecular Complexes). The [11,20-13C2]retinal was a kind gift of Johan Lugtenburg, Peter Verdegem (Leiden University), Neville McLean, Malcolm H. Levitt and Richard C. D. Brown (Southampton University). P.G. gratefully acknowledges financial support by the Lichtenberg program of the Volkswagen Foundation and by a Grant-in-Aid for Scientific Research of the Japan Society for the Promotion of Science.

  2. ‡

    These authors contributed equally to this work.

Publication History

  1. Issue published online: 7 DEC 2011
  2. Article first published online: 27 OCT 2011
  3. Manuscript Received: 9 AUG 2011

Funded by

  • Deutsche Forschungsgemeinschaft. Grant Number: DO545/7-1
  • European Drug Initiative on Channels and Transporters (EDICT). Grant Number: HEALTH-F4-2007-201924
  • NIH. Grant Number: U54 GM094608
  • Center for Biomolecular Magnetic Resonance (BMRZ)
  • Cluster of Excellence Frankfurt
  • Volkswagen Foundation
  • Japan Society for the Promotion of Science

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Keywords:

  • Membranproteine;
  • NMR-Spektroskopie;
  • Proteorhodopsin;
  • Strukturbiologie
Thumbnail image of graphical abstract

Ein gelöstes Rätsel: Die mit Lösungs-NMR-Spektroskopie bestimmte Struktur der aus sieben transmembranären Helices gebildeten Protonenpumpe Proteorhodopsin wurde durch Kombination von Daten aus NOE-Experimenten und verstärkter paramagnetischer Relaxation erhalten (siehe Bild). Die Genauigkeit, mit der die Struktur aufgeklärt wurde, konnte durch dipolare Restkopplungen verbessert werden.

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