Enzymatic Total Synthesis of Defucogilvocarcin M and Its Implications for Gilvocarcin Biosynthesis

Authors

  • Dr. Pallab Pahari,

    1. Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, KY 40536-0596 (USA)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Prof. Dr. Madan K. Kharel,

    1. Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, KY 40536-0596 (USA)
    2. Midway College School of Pharmacy, 120 Scott Perry Drive, Paintsville, KY 41240 (USA)
    Search for more papers by this author
  • Dr. Micah D. Shepherd,

    1. Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, KY 40536-0596 (USA)
    2. zuChem Inc., 801 W. Main Street, Peoria, IL 61606-1877 (USA)
    Search for more papers by this author
  • Prof. Dr. Steven G. van Lanen,

    1. Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, KY 40536-0596 (USA)
    Search for more papers by this author
  • Prof. Dr. Jürgen Rohr

    Corresponding author
    1. Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, KY 40536-0596 (USA)
    • Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, KY 40536-0596 (USA)
    Search for more papers by this author

  • This work was supported by grants CA102102 and CA091901 from the U.S. National Institutes of Health to J.R. The mass spectrometry and NMR facilities of the University of Kentucky are acknowledged for their services.

Abstract

original image

Teamwork: Defucogilvocarcin M (1, siehe Schema) wurde in einer Eintopfreaktion unter Einwirkung von 15 Enzymen aus E. coli und den Gilvocarcin-, Jadomycin- und Ravidomycin-Biosynthesewegen aus Acetyl-CoA und Malonyl-CoA synthetisiert. Die Enzymmischung wurde systematisch vereinfacht und variiert, um die komplexen Schritte der späten Gilvocarcin-Biosynthese zu beleuchten.

Ancillary