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Photocontrollable Dynamic Micropatterning of Non-adherent Mammalian Cells Using a Photocleavable Poly(ethylene glycol) Lipid

Authors

  • Dr. Satoshi Yamaguchi,

    Corresponding author
    1. Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
    • Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
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  • Shinya Yamahira,

    1. Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
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  • Kyoko Kikuchi,

    1. Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565 (Japan)
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  • Kimio Sumaru,

    1. Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565 (Japan)
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  • Toshiyuki Kanamori,

    1. Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565 (Japan)
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  • Prof. Teruyuki Nagamune

    Corresponding author
    1. Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
    2. Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
    • Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)
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  • This work was supported by the Center for NanoBio Integration in The University of Tokyo.

Abstract

original image

Zellen im Scheinwerferlicht: Ein Substrat wurde mit einem Polyethylenglycol(PEG)-Segment bedeckt, das über einen Linker an ein Lipid gebunden war. Durch UV-Bestrahlung in beliebigen Mustern wurde der Linker gespalten, und eine PEG-bedeckte Fläche blieb zurück, sodass kontrastreiche Muster von Zellen einfach erhalten wurden, von denen selektiv die angestrahlten Zellen unbeschädigt in einem Mikrofluidikaufbau abgelöst werden können (siehe Bild).

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