Deep-Tissue Photoacoustic Tomography of a Genetically Encoded Near-Infrared Fluorescent Probe

Authors

  • Dr. Grigory S. Filonov,

    1. Department of Anatomy and Structural Biology, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461 (USA)
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    • These authors contributed equally to this work.

  • Arie Krumholz,

    1. Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University in St. Louis, One Brookings Drive, St. Louis, MO 63130 (USA)
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    • These authors contributed equally to this work.

  • Dr. Jun Xia,

    1. Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University in St. Louis, One Brookings Drive, St. Louis, MO 63130 (USA)
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  • Junjie Yao,

    1. Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University in St. Louis, One Brookings Drive, St. Louis, MO 63130 (USA)
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  • Dr. Lihong V. Wang,

    Corresponding author
    1. Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University in St. Louis, One Brookings Drive, St. Louis, MO 63130 (USA)
    • Department of Biomedical Engineering, Optical Imaging Laboratory, Washington University in St. Louis, One Brookings Drive, St. Louis, MO 63130 (USA)
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  • Dr. Vladislav V. Verkhusha

    Corresponding author
    1. Department of Anatomy and Structural Biology, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461 (USA)
    • Department of Anatomy and Structural Biology, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461 (USA)
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  • We thank Bojana Gligorijevic for help with the mice, and Jeffrey Segall for providing the EGFP-expressing MTLn3 cells. We are grateful to Benjamin Glick (University of Chicago), Dmitry Chudakov and Konstantin Lukyanov (both from the Institute of Bioorganic Chemistry, Russia) for the plasmids encoding GFP-like proteins. We thank Yu Zhang and Yunan Xia for help with maintaining cell cultures. We thank James Ballard for help with editing the manuscript. This work was supported by the National Institutes of Health grants GM073913 and CA164468 (to V.V.V.), and in part by EB000712, EB008085, CA134539, and CA136398 (to L.V.W.).

Abstract

original image

Unter die Haut: Ein im nahen Infrarot fluoreszierendes Protein (iRFP) zeigt überlegene Eigenschaften in der photoakustischen Gewebetomographie. Bei einer Gewebetiefe von 4 mm im lebenden Tier werden laterale und axiale Auflösungen von 280 bzw. 75 μm erzielt. Dies ermöglicht volumetrische Aufnahmen eines Tumors (siehe Bild), wodurch die räumlich aufgelöste Verfolgung der Tumorentwicklung möglich wird.

Ancillary