Zuschrift

Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad-Spectrum Nanoquencher†

  1. Dr. Xinglu Huang1,
  2. Magdalena Swierczewska1,2,
  3. Dr. Ki Young Choi1,
  4. Dr. Lei Zhu1,
  5. Dr. Ashwinkumar Bhirde1,
  6. Dr. Jinwoo Park3,
  7. Dr. Kwangmeyung Kim4,
  8. Prof. Jin Xie5,
  9. Dr. Gang Niu1,
  10. Prof. Kang Choon Lee6,
  11. Dr. Seulki Lee1,*,
  12. Dr. Xiaoyuan Chen1,*

Article first published online: 2 JAN 2012

DOI: 10.1002/ange.201107795

Issue

Angewandte Chemie

Angewandte Chemie

Volume 124, Issue 7, pages 1657–1662, February 13, 2012

Additional Information

How to Cite

Huang, X., Swierczewska, M., Choi, K. Y., Zhu, L., Bhirde, A., Park, J., Kim, K., Xie, J., Niu, G., Lee, K. C., Lee, S. and Chen, X. (2012), Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad-Spectrum Nanoquencher. Angew. Chem., 124: 1657–1662. doi: 10.1002/ange.201107795

Author Information

  1. 1

    Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)

  2. 2

    Department of Biomedical Engineering, Stony Brook University (USA)

  3. 3

    BioActs, DKC Corporation (Korea)

  4. 4

    Center for Theragnosis, Korea Institute of Science and Technology (KIST, Korea)

  5. 5

    Department of Chemistry and Bio-Imaging Research Center, University of Georgia (USA)

  6. 6

    College of Pharmacy, SungKyunKwan University (Korea)

*Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)

  1. †

    This work was supported by the Intramural Research Program of the NIBIB, NIH and partially supported by an NIH Pathway to Independence (K99/R00) Award, the Henry M. Jackson Foundation, and the Intramural Research Program of KIST (Korea). We thank Myung Sun Lee for illustrations.

Publication History

  1. Issue published online: 7 FEB 2012
  2. Article first published online: 2 JAN 2012
  3. Manuscript Revised: 13 DEC 2011
  4. Manuscript Received: 5 NOV 2011

Funded by

  • NIBIB, NIH
  • NIH
  • Henry M. Jackson Foundation
  • KIST

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Keywords:

  • Caspasen;
  • Fluoreszenz;
  • Imaging-Substanzen;
  • Nanopartikel;
  • Proteolyse
Thumbnail image of graphical abstract

Licht aus! Ein universeller Nanoquencher, der eine große Bandbreite von Farbstoffen für den sichtbaren und Nah-IR-Bereich löscht, funktioniert über eine Serie von Dunkelquenchern, die in ein zellgängiges mesoporöses Siliciumoxidteilchen eingebaut sind. Kombiniert mit farbstoffmarkierten Substraten erzeugt der Nanoquencher mehrfache Fluoreszenzsignale bei spezifischer Proteolyse, was die Abbildung proteolytischer Kaskaden in Echtzeit ermöglicht (siehe Schema).

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