Design, Total Chemical Synthesis, and X-Ray Structure of a Protein Having a Novel Linear-Loop Polypeptide Chain Topology

Authors

  • Kalyaneswar Mandal,

    1. Department of Biochemistry and Molecular Biology, Department of Chemistry, Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637 (USA)
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  • Brad L. Pentelute,

    1. Department of Biochemistry and Molecular Biology, Department of Chemistry, Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637 (USA)
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  • Duhee Bang,

    1. Department of Biochemistry and Molecular Biology, Department of Chemistry, Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637 (USA)
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  • Zachary P. Gates,

    1. Department of Biochemistry and Molecular Biology, Department of Chemistry, Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637 (USA)
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  • Vladimir Yu. Torbeev,

    1. Department of Biochemistry and Molecular Biology, Department of Chemistry, Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637 (USA)
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  • Prof. Stephen B. H. Kent

    Corresponding author
    1. Department of Biochemistry and Molecular Biology, Department of Chemistry, Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637 (USA)
    • Department of Biochemistry and Molecular Biology, Department of Chemistry, Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637 (USA)
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  • This research was supported by the Office of Science (BER), U.S. Department of Energy (grant no. DE-FG02-07ER64501 to S.B.H.K.).

Abstract

original image

Geschlossener Kreis: Neuartige Methoden zur Synthese und Strukturaufklärung wurden genutzt, um ein Proteinmolekül mit bisher beispielloser Linear-Loop-Topologie der Polypeptidkette herzustellen und dessen Röntgenkristallstruktur zu charakterisieren.

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