Directing Stem Cell Fate by Controlling the Affinity and Density of Ligand–Receptor Interactions at the Biomaterials Interface

Authors

  • Prof. Kristopher A. Kilian,

    1. Department of Chemistry, Department of Cell and Molecular Biology, Department of Biomedical Engineering, Northwestern University (USA)
    2. Current address: Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign (USA)
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  • Prof. Milan Mrksich

    Corresponding author
    1. Department of Chemistry, Department of Cell and Molecular Biology, Department of Biomedical Engineering, Northwestern University (USA)
    • Department of Chemistry, Department of Cell and Molecular Biology, Department of Biomedical Engineering, Northwestern University (USA)
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  • This work was supported by the National Cancer Institute of the National Institutes of Health. K.A.K. was supported by a Ruth L. Kirschstein National Research Service Award Number F32M087048 from the National Institute of General Medical Sciences of the National Institutes of Health.

Abstract

original image

Die Differenzierung mesenchymaler Stammzellen kann durch die Affinität und Dichte eines immobilisierten Liganden für den Integrinrezeptor beeinflusst werden. Zellen an Monoschichten, die das hochaffine cyclische RGD-Peptid (links) präsentieren, zeigen eine erhöhte Expression von osteogenen Markern, während Zellen an Monoschichten, die das weniger affine lineare RGD-Peptid (rechts) präsentieren, frühe myogene Marker bei hoher Ligandendichte und neurogene Marker bei niedriger Ligandendichte exprimieren.

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