Discovery and Target Identification of an Antiproliferative Agent in Live Cells Using Fluorescence Difference in Two-Dimensional Gel Electrophoresis

Authors

  • Jongmin Park,

    1. Department of Chemistry, College of Natural Science, Seoul National University, Seoul 151-747 (Korea)
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  • Dr. Sangmi Oh,

    1. Department of Chemistry, College of Natural Science, Seoul National University, Seoul 151-747 (Korea)
    2. Current address: Medicinal Chemistry and Chemical Biology Group, Institut Pasteur Korea, Gyeonggi 463-400 (Korea)
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  • Prof. Seung Bum Park

    Corresponding author
    1. Department of Chemistry, College of Natural Science, Seoul National University, Seoul 151-747 (Korea)
    2. Department of Biophysics and Chemical Biology/Bio-MAX institute Seoul National University, Seoul 151-747 (Korea)
    • Department of Chemistry, College of Natural Science, Seoul National University, Seoul 151-747 (Korea)
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  • This work was supported by a Chemical Genomics Research grant (NRF-2011-0000058), a Global Frontier Project grant (NRF-M1AXA002-0032150), and the World Class University program (R31-2010-000-10032-0) of the National Research Foundation, funded by the Ministry of Education, Science, and Technology of Korea. J.P. received a BK21 Scholarship and a Seoul Science Fellowship.

Abstract

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Ziel gefunden: Durch Fluoreszenzdifferenz in der 2D-Gelelektrophorese (FITGE) können Wechselwirkungen zwischen Proteinen und Wirkstoffmolekülen in intakter zellulärer Umgebung beobachtet werden. Wo konventionelle Methoden keinen Erfolg hatten, gelang mit FITGE die Identifizierung des Zielproteins einer antiproliferativen Verbindung in lebenden Zellen durch Differenzierung zwischen spezifischer und unspezifischer Bindung von Photoaffinitätssonden.

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