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Creation of an α-Mannosynthase from a Broad Glycosidase Scaffold

Authors

  • Keisuke Yamamoto,

    1. Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA (UK)
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  • Prof. Benjamin G. Davis

    Corresponding author
    1. Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA (UK)
    • Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA (UK)
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  • The authors thank their sources of financial support: VTU (http://www.vtu.com/) and B.G.D. is also a recipient of a Royal Society Wolfson Research Merit Award. We thank Mitul Patel for preparation of 2,3,4,6-tetra-O-acetyl β-mannosyl fluoride, Dr. Tim Claridge for assistance with NMR spectroscopy analysis and Drs. Thomas Purkarthofer, Roland Weis, and Iskandar Dib at VTU for useful discussions.

Abstract

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α-Mannoside synthetisierende Biokatalysatoren wurden durch Mutation einer α-Glucosidase der GH31-Familie erhalten. Diese zeigt Plastizität gegenüber Veränderungen an der 2-OH-Position von Donorsubstraten. Ein Fluorid-Donorreagens ergab mithilfe dieser α-Mannosynthase, die eine niedrige (unerwünschte) Oligomerisierungsaktivität aufweist, mehrere mono-α-mannosylierte Konjugate.

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