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In Situ Solid-State NMR Spectroscopy of Protein in Heterogeneous Membranes: The Baseplate Antenna Complex of Chlorobaculum tepidum

Authors

  • M. Sc. Natalia V. Kulminskaya,

    1. Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, University of Aarhus, Langelandsgade 140, 8000 Aarhus C (Denmark)
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  • Dr. Marie Ø. Pedersen,

    1. Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, University of Aarhus, Langelandsgade 140, 8000 Aarhus C (Denmark)
    2. Novo Nordisk A/S, Måløv (Denmark)
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  • Dr. Morten Bjerring,

    1. Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, University of Aarhus, Langelandsgade 140, 8000 Aarhus C (Denmark)
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  • Dr. Jarl Underhaug,

    1. Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, University of Aarhus, Langelandsgade 140, 8000 Aarhus C (Denmark)
    2. Department of Biomedicine, University of Bergen (Norway)
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  • Assoc. Prof. Dr. Mette Miller,

    1. Department of Biochemistry and Molecular Biology, University of Southern Denmark (Denmark)
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  • Assoc. Prof. Dr. Niels-Ulrik Frigaard,

    1. Department of Biology, University of Copenhagen (Denmark)
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  • Dr. Jakob T. Nielsen,

    Corresponding author
    1. Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, University of Aarhus, Langelandsgade 140, 8000 Aarhus C (Denmark)
    • Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, University of Aarhus, Langelandsgade 140, 8000 Aarhus C (Denmark)
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  • Prof. Dr. Niels Chr. Nielsen

    Corresponding author
    1. Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, University of Aarhus, Langelandsgade 140, 8000 Aarhus C (Denmark)
    • Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, University of Aarhus, Langelandsgade 140, 8000 Aarhus C (Denmark)
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  • We acknowledge support from the Danish National Research Foundation, the Danish Centre for Scientific Computing, and the BIONMR FP7 program.

Abstract

original image

Eine clevere Kombination: CsmA-Proteine in der heterogenen Umgebung des Chlorobaculum-tepidum-Photorezeptors wurden durch In-situ-Festkörper-NMR-Spektroskopie untersucht. Durch Kombination von 2D- und 3D-NMR-Spektren können 90 % der CsmA-Resonanzen zugeordnet werden, die basierend auf chemischen Verschiebungsdaten Informationen über die Struktur und Konformation von CsmA im CsmA-Bacteriochlorophyll-a-Komplex liefern (siehe Bild).

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