Merging of Confocal and Caging Technologies: Selective Three-Color Communication with Profluorescent Reporters

Authors

  • Prof. Melanie A. Priestman,

    1. Department of Chemistry, Division of Chemical Biology and Medicinal Chemistry, and Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599 (USA)
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  • Dr. Thomas A. Shell,

    1. Department of Chemistry, Division of Chemical Biology and Medicinal Chemistry, and Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599 (USA)
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  • Dr. Liang Sun,

    1. Pharmaron-Beijing BDA, Beijing, 100176 (P.R. China)
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  • Prof. Hsien-Ming Lee,

    1. Institute of Chemistry, Academia Sinica, Nankang Dist. Taipei, 115 (Taiwan)
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  • Prof. David S. Lawrence

    Corresponding author
    1. Department of Chemistry, Division of Chemical Biology and Medicinal Chemistry, and Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599 (USA)
    • Department of Chemistry, Division of Chemical Biology and Medicinal Chemistry, and Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599 (USA)
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  • We thank the NIH (GM086217) for financial support.

Abstract

original image

Spaltung auf Kommando: Signalwege haben oft eine wichtige Raum-Zeit-Komponente, die sich am besten mit lichtaktivierbaren Reagentien untersuchen lässt. Drei separate photolabile Einheiten, die auf Licht unterschiedlicher Wellenlänge (360, 440 bzw. 560 nm) reagieren, wurden synthetisiert und geprüft. Das Dreifarbensystem wurde in Bildgebungsstudien an Mikrovertiefungen und in HeLa-Zellen eingesetzt (siehe Bild).

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