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A Magnetic Gram Stain for Bacterial Detection

Authors

  • Dr. Ghyslain Budin,

    1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114 (USA)
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    • These authors contributed equally to this work.

  • Dr. Hyun Jung Chung,

    1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114 (USA)
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    • These authors contributed equally to this work.

  • Prof. Hakho Lee,

    1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114 (USA)
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  • Prof. Ralph Weissleder

    Corresponding author
    1. Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114 (USA)
    2. Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115 (USA)
    • Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114 (USA)
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  • This work was supported by the National Institutes of Health (NIH) grant number 2P50CA086355. We thank Yoshi Iwamoto and Alex Zaltsman for image processing.

Abstract

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Magnetisierend: Bakterien werden durch Färbung mit Kristallviolett (CV) oft in Gram-positive und Gram-negative Stämme klassifiziert. Die bioorthogonale Modifikation von CV mit trans-Cycloocten kann zur Magnetisierung Gram-positiver Bakterien mit Tetrazin-funktionalisierten magnetischen Nanopartikeln (MNP-Tz) genutzt werden. Die Methode ermöglicht die klassenspezifische automatische magnetische Detektion und Trennung von Bakterien.

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