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Converting a Solvatochromic Fluorophore into a Protein-Based pH Indicator for Extreme Acidity

Authors

  • Maiyun Yang,

    1. Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Department of Chemical Biology, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
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    • These authors contributed equally to this work.

  • Yanqun Song,

    1. Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Department of Chemical Biology, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
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    • These authors contributed equally to this work.

  • Dr. Meng Zhang,

    1. Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Department of Chemical Biology, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
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  • Shixian Lin,

    1. Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Department of Chemical Biology, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
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  • Ziyang Hao,

    1. Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Department of Chemical Biology, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
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  • Yuan Liang,

    1. Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Department of Chemical Biology, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
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  • Dianmu Zhang,

    1. Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Department of Chemical Biology, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
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  • Prof. Dr. Peng R. Chen

    Corresponding author
    1. Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Department of Chemical Biology, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
    • Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Department of Chemical Biology, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
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  • This work was supported by research grants from the National Key Basic Research Foundation of China (grant numbers 2010CB912300 and 2012CB917300), the National Natural Science Foundation of China (grant numbers 91013005 and 21001010), and Peking University.

Abstract

original image

pH-Messung in lebenden Zellen: Ein umgebungsempfindlicher Fluorophor (grün) wurde ortsspezifisch an ein säureresistentes Chaperon (HdeA) konjugiert, das bei niedrigem pH seine Konformation ändert. Eine Analyse der Konjugationsstellen führte zur Identifizierung einer HdeA-Stelle, an der das verknüpfte Fluorophor beim Ansäuern einen starken Fluoreszenzanstieg zeigt.

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