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Genetic Incorporation of a Metal-Chelating Amino Acid as a Probe for Protein Electron Transfer

Authors

  • Xiaohong Liu,

    1. Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing, 100101 (China)
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    • These authors contributed equally to this work.

  • Jiasong Li,

    1. Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing, 100101 (China)
    2. School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230026 (China)
    3. Graduate School of the Chinese Academy of Sciences, Beijing, 100864 (China)
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    • These authors contributed equally to this work.

  • Jianshu Dong,

    1. Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing, 100101 (China)
    2. Graduate School of the Chinese Academy of Sciences, Beijing, 100864 (China)
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    • These authors contributed equally to this work.

  • Cheng Hu,

    1. Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing, 100101 (China)
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  • Weimin Gong,

    Corresponding author
    1. Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing, 100101 (China)
    • Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing, 100101 (China)
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  • Jiangyun Wang

    Corresponding author
    1. Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing, 100101 (China)
    • Laboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing, 100101 (China)
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  • We gratefully acknowledge the Major State Basic Research Program of China (2010CB912301, 2009CB825505), National Science Foundation of China (90913022, 31100616, 30900231), CAS grants (KZCX2-YW-JC101 and KSCX2-EW-G-7) to J.Y.W., W.M.G., and X.H.L. We thank Z. Xie and X. Peng for help with protein mass spectrometry and Y. Wang with fluorescence lifetime determination. We thank Prof. X. Zhao and Y. Wong for helpful discussions.

Abstract

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ET trifft Qualle: Die Einführung der Metallionen chelatisierenden Aminosäure pyTyr in Grün fluoreszierendes Protein (GFP) aus Quallen ermöglicht einen abstandsabhängigen photoinduzierten Elektronentransfer (ET) vom GFP-Chromophor auf ein gebundenes CuII-Ion binnen einer Nanosekunde. Eine Kristallstruktur von GFP mit pyTyr an einer bestimmten Position offenbart die strukturelle Grundlage für die nanomolare Bindungsaffinität von pyTyr für CuII-Ionen.

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