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Genomics-Driven Discovery of Burkholderic Acid, a Noncanonical, Cryptic Polyketide from Human Pathogenic Burkholderia Species

Authors

  • Jakob Franke,

    1. Dept. of Biomolecular Chemistry, Leibniz Institute for Natural Product Research and Infection Biology, HKI, Beutenbergstr. 11a, 07745 Jena (Germany)
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    • These authors contributed equally to this work.

  • Dr. Keishi Ishida,

    1. Dept. of Biomolecular Chemistry, Leibniz Institute for Natural Product Research and Infection Biology, HKI, Beutenbergstr. 11a, 07745 Jena (Germany)
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    • These authors contributed equally to this work.

  • Prof. Dr. Christian Hertweck

    Corresponding author
    1. Dept. of Biomolecular Chemistry, Leibniz Institute for Natural Product Research and Infection Biology, HKI, Beutenbergstr. 11a, 07745 Jena (Germany)
    2. Friedrich Schiller University, Jena (Germany)
    • Dept. of Biomolecular Chemistry, Leibniz Institute for Natural Product Research and Infection Biology, HKI, Beutenbergstr. 11a, 07745 Jena (Germany)
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  • We thank A. Perner and H. Heineke for MS and NMR spectroscopy measurements, Dr. H. M. Dahse for preliminary biological assays and Dr. S. Pidot for critically reading the mansucript. This work was supported by the “Pakt für Forschung und Innovation” of the Free State of Thuringia and the BMBF, and the International Leibniz Research School for Biomolecular and Microbial Interactions (ILRS), as part of the excellence graduate school Jena School for Microbial Communication (JSMC).

Abstract

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Geheimnis gelüftet: Der Promotoraustausch in einem kryptischen Biosynthese-Gencluster, das in bestimmten pathogenen Burkholderia-Arten konserviert ist, führte zu einem instabilen, strukturell beispiellosen Polyketid (1). Markierungs- und Geninaktivierungs-Experimente sowie Bioinformatik-Analysen liefern erste Erkenntnisse zu einem faszinierenden Polyketid-Syntheseweg.

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