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Generation of Controllable Gradients in Cell Density

Authors

  • Wenying Liu,

    1. School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332 (USA)
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  • Yu Zhang,

    1. The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332 (USA)
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  • Prof. Stavros Thomopoulos,

    1. Department of Orthopaedic Surgery, Washington University, School of Medicine, St. Louis, MO 63110 (USA)
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  • Prof. Younan Xia

    Corresponding author
    1. The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, School of Chemistry and Biochemistry and School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332 (USA)
    • The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, School of Chemistry and Biochemistry and School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332 (USA)

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  • This work was supported in part by a grant from the NIH (1R01 AR060820) and startup funds from the Georgia Institute of Technology.

Abstract

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Ungleich verteilt: Ein Zelldichtegradient entsteht, wenn man ein Substrat in einem bestimmten Winkel in eine homogene Zellsuspension einhängt, weil sich die Zahl der zur Absetzung befähigten Zellen nach und nach ändert (siehe Schema). Inverse Gradienten wurden auf demselben Substrat durch Anwendung mehrerer Absetzungsprozeduren erhalten.

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