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Simultaneous Measurement of SUMOylation using SNAP/CLIP-Tag-Mediated Translation at the Single-Molecule Level

Authors

  • Dr. Yong Yang,

    1. Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong, 518055 (China)
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  • Prof. Dr. Chun-yang Zhang

    Corresponding author
    1. Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong, 518055 (China)
    • Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong, 518055 (China)
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  • This work was supported by the National Basic Research Program 973 (Grant Nos. 2011CB933600 and 2010CB732600), the Award for the Hundred Talent Program of the Chinese Academy of Science, the Natural Science Foundation of China (Grant Nos. 21075129 and 31000599), the Guangdong Innovation Research Team Fund for Low-cost Healthcare Technologies, the Natural Science Foundation of Shenzhen City (Grant Nos. JC201005270327A and JC201005270355A), and the Fund for Shenzhen Engineering Laboratory of Single-molecule Detection and Instrument Development (Grant No. (2012) 433). SUMO=small ubiquitin-like modifier.

Abstract

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Interesse an SUMO: Eine gleichzeitige Messung mehrfacher SUMOylierungen gelang auf dem Einzelmolekülniveau durch Kombination von SNAP/CLIP-Tag-vermittelter Translation mit Einzelmoleküldetektion (siehe Schema; SUMO=small ubiquitin-like modifier). Die Methode ist außerordentlich empfindlich und ermöglicht die Messung der SUMOylierung verschiedener Proteine unter einer Reihe physiologischer Bedingungen.

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