Get access

A Plasmonic DNAzyme Strategy for Point-of-Care Genetic Detection of Infectious Pathogens

Authors

  • Kyryl Zagorovsky,

    1. Institute of Biomaterials and Biomedical Engineering, Terrence Donnelly Centre for Cellular and Biomolecular Research, Chemistry, Chemical Engineering, Materials Science and Engineering, University of Toronto, 160 College Street, Room 450, Toronto, ON, M5S3E1 (Canada)
    Search for more papers by this author
  • Dr. Warren C. W. Chan

    Corresponding author
    1. Institute of Biomaterials and Biomedical Engineering, Terrence Donnelly Centre for Cellular and Biomolecular Research, Chemistry, Chemical Engineering, Materials Science and Engineering, University of Toronto, 160 College Street, Room 450, Toronto, ON, M5S3E1 (Canada)
    • Institute of Biomaterials and Biomedical Engineering, Terrence Donnelly Centre for Cellular and Biomolecular Research, Chemistry, Chemical Engineering, Materials Science and Engineering, University of Toronto, 160 College Street, Room 450, Toronto, ON, M5S3E1 (Canada)

    Search for more papers by this author

  • The authors acknowledge the Canadian Institute of Health Research (MOP93532) and Natural Science and Engineering Research Council (CRDPG411601) for funding through the CHRP program (CPG-112321), Canadian Foundation for Innovation, and Ministry of Research and Innovation. The authors thank Mr. Leo Chou for pertinent discussions, and Dr. Anu Rebbapragada and Mr. Stephen Perusini for designing the targets for N. gonorrhoeae detection.

Abstract

original image

Schnell erkannt: Eine DNAzym-Signalverstärkung (siehe Schema) wurde mit Goldnanopartikeln (GNPs) kombiniert, um einen einfachen und empfindlichen kolorimetrischen Assay für verschiedene genetische Zielstrukturen zu erhalten. Der Assay hat eine Empfindlichkeit von 50 pM, erfordert keine Reinigungsschritte und kann mehrere Zielstrukturen parallel detektieren. Infektionen mit Gonorrhoe, Syphilis, Malaria und Hepatitis B ließen sich so schnell nachweisen.

Get access to the full text of this article

Ancillary