Two-Way Nanopore Sensing of Sequence-Specific Oligonucleotides and Small-Molecule Targets in Complex Matrices Using Integrated DNA Supersandwich Structures

Authors

  • Nannan Liu,

    1. School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074 (P.R. China)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Yanan Jiang,

    1. School of Chemistry and Environment, Beijing University of Aeronautics and Astronautics, Beijing 100191 (P.R. China)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Yahong Zhou,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 (P.R. China)
    Search for more papers by this author
  • Prof. Fan Xia,

    Corresponding author
    1. School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074 (P.R. China)
    • School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074 (P.R. China)
    Search for more papers by this author
  • Dr. Wei Guo,

    Corresponding author
    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 (P.R. China)
    • Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 (P.R. China)
    Search for more papers by this author
  • Prof. Lei Jiang

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 (P.R. China)
    2. School of Chemistry and Environment, Beijing University of Aeronautics and Astronautics, Beijing 100191 (P.R. China)
    Search for more papers by this author

  • We thank Prof. Lin Feng (THU), Dr. Liuxuan Cao (PKU), and Dr. Kang Sun (ICCAS) for beneficial discussions. This work is supported by the National Research Fund for Fundamental Key Projects (2011CB935700, 2012CB933200, 2013CB933003, 2013CB933000 2010CB934700, and 2009CB930404) and the National Natural Science Foundation of China (21103201, 11290163, 91127025, 20920102036, 20974113, and 21121001).

Abstract

original image

Subnanomolar und sequenzspezifisch gelingt der DNA-Nachweis und submikromolar der von niedermolekularen Verbindungen (ATP) über die Selbstorganisation und Zerlegung von DNA-Überstrukturen in Festkörper-Nanoporen (siehe Schema). Der in diese DNA-Strukturen eingebaute Verstärkungsmechanismus führt zu größerer Signalstärke und Empfindlichkeit. Der Sensor funktionierte auch in komplexen Gemischen wie Säuger-Serum.

Ancillary