A Facile Strategy for Selective Incorporation of Phosphoserine into Histones

Authors

  • Sangsik Lee,

    1. Department of Chemistry, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)
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  • Seunghee Oh,

    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)
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  • Aerin Yang,

    1. Department of Chemistry, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)
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  • Jihyo Kim,

    1. Department of Chemistry, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)
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  • Prof. Dieter Söll,

    1. Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Ave, New Haven, CT 06511 (USA)
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  • Prof. Daeyoup Lee,

    Corresponding author
    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)
    • Daeyoup Lee, Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)

      Hee-Sung Park, Department of Chemistry, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)

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  • Prof. Hee-Sung Park

    Corresponding author
    1. Department of Chemistry, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)
    • Daeyoup Lee, Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)

      Hee-Sung Park, Department of Chemistry, Korea Advanced Institute of Science and Technology, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701 (Republic of Korea)

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  • This work was supported by grants from the National Research Foundation of Korea (2011-0031363, 2011-0020322, and 2012R1A2A2A02047051 to H.-S.P.), the Stem Cell Research Program (2012M 3A9B 4027953 to D.L.), and DARPA (Contract N660-12-C-4211 to D.S.) and NIH (GM22854 to D.S.).

Abstract

original image

Einbau von Phosphoserin: Ein allgemeiner Weg zu rekombinanten Histonen mit ortsspezifischer Serin-Phosphorylierung nutzt das Engineering einer Phosphoseryl-tRNA-Synthetase (SepRS) und eines Elongationsfaktors Tu (EF-Tu; siehe Bild). Serin-phosphorylierte Nucleosome sind ein direkter Beweis für die gegenseitige Beeinflussung von Phosphorylierung und Acetylierung in Histonen.

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