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High-Yield Production of Dihydrogen from Xylose by Using a Synthetic Enzyme Cascade in a Cell-Free System

Authors

  • Julia S. Martín del Campo,

    1. Biological Systems Engineering Department, Virginia Tech, Blacksburg, VA 24061 (USA)
    2. Departamento de Física Aplicada, Centro de Investigación y de Estudios Avanzados-Merida, Carretera antigua a Progreso Km. 6, A.P. 73 Cordemex, 97310, Mérida, Yucatán (México)
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  • Joseph Rollin,

    1. Biological Systems Engineering Department, Virginia Tech, Blacksburg, VA 24061 (USA)
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  • Suwan Myung,

    1. Biological Systems Engineering Department, Virginia Tech, Blacksburg, VA 24061 (USA)
    2. Institute for Critical Technology and Applied Sciences (ICTAS), Virginia Tech, Blacksburg, VA 24061 (USA)
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  • You Chun,

    1. Biological Systems Engineering Department, Virginia Tech, Blacksburg, VA 24061 (USA)
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  • Sanjeev Chandrayan,

    1. Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 (USA)
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  • Prof. Rodrigo Patiño,

    1. Departamento de Física Aplicada, Centro de Investigación y de Estudios Avanzados-Merida, Carretera antigua a Progreso Km. 6, A.P. 73 Cordemex, 97310, Mérida, Yucatán (México)
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  • Prof. Michael WW Adams,

    1. Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 (USA)
    2. DOE BioEnergy Science Center (BESC), Oak Ridge, TN 37831 (USA)
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  • Prof.  Y.-H. Percival Zhang

    Corresponding author
    1. Biological Systems Engineering Department, Virginia Tech, Blacksburg, VA 24061 (USA)
    2. Institute for Critical Technology and Applied Sciences (ICTAS), Virginia Tech, Blacksburg, VA 24061 (USA)
    3. DOE BioEnergy Science Center (BESC), Oak Ridge, TN 37831 (USA)
    4. Cell-Free Bioinnovations Inc., Blacksburg, VA 24060 (USA)
    • Biological Systems Engineering Department, Virginia Tech, Blacksburg, VA 24061 (USA)

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  • This work was supported by the Biological Systems Engineering Department of Virginia Tech, and partially supported by Shell GameChanger Program, the CALS Biodesign and Bioprocessing Research Center, and the DOE BioEnergy Science Center (to P.Z.) and the Division of Chemical Sciences, Geosciences and Biosciences, Office of Basic Energy Sciences of the DOE (DE-FG05-95ER20175 to M.A.). JSMC thanked the Mexican Council of Science and Technology (Conacyt) for her PhD grant.

Abstract

original image

13 Enzyme in einem Reaktor (rot im Schema), darunter die neue Polyphosphat-Xylulokinase (XK), wandeln Xylose in H2 und CO2 um, wobei nahezu 100 % der theoretisch möglichen Ausbeute erreicht werden. Die Ergebnisse legen nahe, dass zellfreie Biosysteme ausgehend von Xylose kostengünstig H2 produzieren könnten. Xu5P=Xylulose-5-phosphat, G6P=Glucose-6-phosphat.

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