Titelbild: Expression of Fluorescent Cyclotides using Protein Trans-Splicing for Easy Monitoring of Cyclotide–Protein Interactions (Angew. Chem. 11/2013)

Authors

  • Dr. Krishnappa Jagadish,

    1. Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90033 (USA)
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  • Dr. Radhika Borra,

    1. Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90033 (USA)
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  • Vanessa Lacey,

    1. Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, CA 92037 (USA)
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  • Subhabrata Majumder,

    1. Department of Chemistry, State University of New York, Albany, NY 12222 (USA)
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  • Dr. Alexander Shekhtman,

    1. Department of Chemistry, State University of New York, Albany, NY 12222 (USA)
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  • Dr. Lei Wang,

    1. Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, CA 92037 (USA)
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  • Dr. Julio A. Camarero

    Corresponding author
    1. Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90033 (USA)
    2. Department of Chemistry, University of Southern California, Los Angeles, CA 90033 (USA)
    • Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90033 (USA)

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Abstract

original image

Cyclotide sind natürliche pflanzliche Mikroproteine mit zirkulärer Kopf-Schwanz-Verknüpfung und Cys-Knoten. In ihrer Zuschrift auf S. 3208 ff. beschreiben J. A. Camarero et al. die rekombinative Produktion eines fluoreszenzmarkierten Cyclotids in lebenden Bakterienzellen. Das in Gegenwart von p-Azidophenylalanin und einem Dibenzocyclooctin-Derivat des Fluoreszenzfarbstoffs Aminomethyl-Cumarinacetat (AMCA) synthetisierte Peptid wird in vivo markiert. Bildgestaltung: Isaac Mora.

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