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Rolling Circle Amplification in a Prokaryotic Translation System Using Small Circular RNA

Authors

  • Naoko Abe,

    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)
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  • Dr. Michio Hiroshima,

    1. Cellular Informatics Laboratory, RIKEN Advanced Science Institute (Japan)
    2. Laboratory for Cell Signaling Dynamics, RIKEN Quantitative Biology Center (Japan)
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  • Hideto Maruyama,

    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)
    2. Faculty of Pharmaceutical Sciences, Hokkaido University (Japan)
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  • Dr. Yuko Nakashima,

    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)
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  • Dr. Yukiko Nakano,

    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)
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  • Prof. Dr. Akira Matsuda,

    1. Faculty of Pharmaceutical Sciences, Hokkaido University (Japan)
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  • Prof. Dr. Yasushi Sako,

    1. Cellular Informatics Laboratory, RIKEN Advanced Science Institute (Japan)
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  • Prof. Dr. Yoshihiro Ito,

    Corresponding author
    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)
    • Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)

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  • Prof. Dr. Hiroshi Abe

    Corresponding author
    1. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)
    2. PRESTO (Japan) Science and Technology Agency, 4-1-8, Honcho, Kawaguchi, Saitama 332-0012 (Japan)
    • Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1, Hirosawa, Wako-Shi, Saitama 351-0198 (Japan)

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  • H.A. and Y.I. were supported financially by the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT). This work was also supported by a Grant-in-Aid for Scientific Research on Priority Areas, “Lifesurveyor”, from MEXT. We are grateful to the Support Unit for Bio-Material Analysis, RIKEN BSI Research Resources Center, for help with the MS analysis.

Abstract

original image

Die bessere Arbeitsbühne: Kleine ringförmige RNA-Moleküle mit einem unendlichen offenen Leserahmen wurden synthetisiert und in einem zellfreien E.-coli-Translationssystem getestet. Eine 126 Nucleotide lange ringförmige RNA lieferte um zwei Größenordnungen mehr Produkt als ihr lineares Analogon, weil ein Ribosom bei dieser kontinuierlichen Peptidsynthese an ringförmiger RNA effektiver als an linearer an der Verlängerung arbeiten kann.

Ancillary