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Mix to Validate: A Facile, Reversible PEGylation for Fast Screening of Potential Therapeutic Proteins In Vivo

Authors

  • Dr. Tae Hyung Kim,

    1. Russell H. Morgan Department of Radiology and Radiological Science, Center for Cancer Nanotechnology Excellence, Center for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University, 400 North Broadway, Baltimore, MD 21231 (USA)
    2. College of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Jangan-gu, Suwon 440-746 (Korea)
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  • Dr. Magdalena Swierczewska,

    1. Institute for NanoBioTechnology, Russell H. Morgan Department of Radiology and Radiological Science, Center for Cancer Nanotechnology Excellence, Johns Hopkins University (USA)
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  • Dr. Yumin Oh,

    1. Russell H. Morgan Department of Radiology and Radiological Science, Center for Cancer Nanotechnology Excellence, Center for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University, 400 North Broadway, Baltimore, MD 21231 (USA)
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  • Dr. AeRyon Kim,

    1. Department of Pathology, Johns Hopkins University (USA)
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  • Prof. Dr. Dong Gyu Jo,

    1. College of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Jangan-gu, Suwon 440-746 (Korea)
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  • Prof. Dr. Jae Hyung Park,

    1. Department of Polymer Science and Engineering, Sungkyunkwan University, Suwon (Korea)
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  • Prof. Dr. Youngro Byun,

    1. College of Pharmacy, Seoul National University, Seoul (Korea)
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  • Prof. Dr. Scheherazade Sadegh-Nasseri,

    1. Department of Pathology, Johns Hopkins University (USA)
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  • Prof. Dr. Martin G. Pomper,

    1. Institute for NanoBioTechnology, Russell H. Morgan Department of Radiology and Radiological Science, Center for Cancer Nanotechnology Excellence, Johns Hopkins University (USA)
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  • Prof. Dr. Kang Choon Lee,

    Corresponding author
    1. College of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Jangan-gu, Suwon 440-746 (Korea)
    • Kang Choon Lee, College of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Jangan-gu, Suwon 440-746 (Korea)

      Seulki Lee, Russell H. Morgan Department of Radiology and Radiological Science, Center for Cancer Nanotechnology Excellence, Center for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University, 400 North Broadway, Baltimore, MD 21231 (USA)

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  • Prof. Dr. Seulki Lee

    Corresponding author
    1. Russell H. Morgan Department of Radiology and Radiological Science, Center for Cancer Nanotechnology Excellence, Center for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University, 400 North Broadway, Baltimore, MD 21231 (USA)
    • Kang Choon Lee, College of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Jangan-gu, Suwon 440-746 (Korea)

      Seulki Lee, Russell H. Morgan Department of Radiology and Radiological Science, Center for Cancer Nanotechnology Excellence, Center for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University, 400 North Broadway, Baltimore, MD 21231 (USA)

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  • This work was supported by the National Institute of Biomedical Imaging and Bioengineering (R00EB013450), the National Cancer Institute (U54CA151838 and T32EB013450), and the National Institute of Allergy and Infectious Diseases (2R01AI063764-06) from the National Institutes of Health and the Converging Research Center Program from the National Research Foundation in Korea (2011K000796).

Abstract

original image

Umgarntes Protein: Die PEGylierung von Proteinen über komplementäre Wechselwirkungen zwischen einer His-Markierung und einem Ni2+-Komplex von Nitrilotriessigsäure (NTA; siehe Bild) – ein gängiges Verfahren in der Proteinforschung – verlängert die Halbwertszeit therapeutischer Proteine in der Blutbahn nach systemischer Verabfolgung. In Tiermodellen verbesserte diese ortsspezifische Modifikation die Wirksamkeit der modifizierten TRAIL-Proteine.

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