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Environment-Sensitive Fluorescent Turn-On Probes Targeting Hydrophobic Ligand-Binding Domains for Selective Protein Detection

Authors

  • Yu-De Zhuang,

    1. Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang-Fu Rd., Hsinchu 30013, Taiwan (ROC)
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    • These authors contributed equally to this work.

  • Po-Yi Chiang,

    1. Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang-Fu Rd., Hsinchu 30013, Taiwan (ROC)
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    • These authors contributed equally to this work.

  • Chia-Wen Wang,

    1. Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang-Fu Rd., Hsinchu 30013, Taiwan (ROC)
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  • Prof. Dr. Kui-Thong Tan

    Corresponding author
    1. Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang-Fu Rd., Hsinchu 30013, Taiwan (ROC)
    2. Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, 101 Sec. 2, Kuang-Fu Rd., Hsinchu 30013, Taiwan (ROC)
    • Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang-Fu Rd., Hsinchu 30013, Taiwan (ROC)
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  • We are grateful to the National Science Council (Grant No.: 100-2113-M007-018-MY2) and Ministry of Education (Grant No.: 101N2011E1), Taiwan (ROC) for financial support.

Abstract

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Bei Bindung Licht an: Eine umgebungsempfindliche „anschaltbare“ Fluoreszenzsonde ermöglicht die selektive Detektion von Enzymen wie auch nichtenzymatischen Proteinen (siehe Schema). Zum Anschalten der Fluoreszenz wird dabei die hydrophobe Ligandbindungsdomäne des Zielproteins angesteuert. Die Methode ist spezifisch für das jeweilige Zielprotein.

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