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Site-Specific Engineering of Chemical Functionalities on the Surface of Live Hepatitis D Virus

Authors

  • Shixian Lin,

    1. Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
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    • These authors contributed equally to this work.

  • Huan Yan,

    1. Graduate program in School of Life Sciences, Peking University, Beijing (China)
    2. National Institute of Biological Sciences, Beijing (China)
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    • These authors contributed equally to this work.

  • Lin Li,

    1. National Institute of Biological Sciences, Beijing (China)
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  • Maiyun Yang,

    1. Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
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  • Bo Peng,

    1. National Institute of Biological Sciences, Beijing (China)
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  • She Chen,

    1. National Institute of Biological Sciences, Beijing (China)
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  • Prof. Dr. Wenhui Li,

    Corresponding author
    1. National Institute of Biological Sciences, Beijing (China)
    • Wenhui Li, National Institute of Biological Sciences, Beijing (China)

      Peng R. Chen, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)

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  • Prof. Dr. Peng R. Chen

    Corresponding author
    1. Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
    • Wenhui Li, National Institute of Biological Sciences, Beijing (China)

      Peng R. Chen, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)

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  • This work was supported by the National Basic Research Program of China (973 Program) (2010CB912302 and 2012CB917301 to P.R.C.; 2011CB812501 to W.-H.L.) and the National Natural Science Foundation of China (21225206 and 91013005 to P.R.C.).

Abstract

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Die Expansion des genetischen Codes gekoppelt mit einem Verfahren zum gezielten Virusaufbau in humanen Hepatozyten lieferte einen intakten menschlichen Hepatitis-D-Virus (HDV) mit einer genetisch kodierten nichtnatürlichen Aminosäure. Die resultierenden HDV-Virione, bei denen jeweils eines von fünf verschiedenen Pyrrolysinanaloga ortsspezifisch in die Oberflächenproteine eingeführt wurde, sind fast Wildtyp-artig lebens- und ansteckungsfähig.

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