Highly Efficient Phase Boundary Biocatalysis with Enzymogel Nanoparticles

Authors

  • Olena Kudina,

    1. Department of Coatings and Polymeric Materials, North Dakota State University, Fargo, ND 58108 (USA)
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  • Andrey Zakharchenko,

    1. Department of Chemistry and Biomolecular Science, Clarkson University, 8 Clarkson Ave., Potsdam, NY 13699 (USA)
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  • Oleksandr Trotsenko,

    1. Department of Chemistry and Biomolecular Science, Clarkson University, 8 Clarkson Ave., Potsdam, NY 13699 (USA)
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  • Dr. Alexander Tokarev,

    1. Department of Chemistry and Biomolecular Science, Clarkson University, 8 Clarkson Ave., Potsdam, NY 13699 (USA)
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  • Dr. Leonid Ionov,

    1. Leibniz-Institut für Polymerforschung Dresden e.V. Hohe Straße 6, 01069 Dresden (Germany)
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  • Georgi Stoychev,

    1. Leibniz-Institut für Polymerforschung Dresden e.V. Hohe Straße 6, 01069 Dresden (Germany)
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  • Nikolay Puretskiy,

    1. Leibniz-Institut für Polymerforschung Dresden e.V. Hohe Straße 6, 01069 Dresden (Germany)
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  • Prof. Scott W. Pryor,

    1. Department of Agricultural and Biosystems Engineering, North Dakota State University, Fargo, ND 58108 (USA)
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  • Prof. Andriy Voronov,

    Corresponding author
    1. Department of Coatings and Polymeric Materials, North Dakota State University, Fargo, ND 58108 (USA)
    • Andriy Voronov, Department of Coatings and Polymeric Materials, North Dakota State University, Fargo, ND 58108 (USA)

      Sergiy Minko, Department of Chemistry and Biomolecular Science, Clarkson University, 8 Clarkson Ave., Potsdam, NY 13699 (USA)

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  • Prof. Sergiy Minko

    Corresponding author
    1. Department of Chemistry and Biomolecular Science, Clarkson University, 8 Clarkson Ave., Potsdam, NY 13699 (USA)
    • Andriy Voronov, Department of Coatings and Polymeric Materials, North Dakota State University, Fargo, ND 58108 (USA)

      Sergiy Minko, Department of Chemistry and Biomolecular Science, Clarkson University, 8 Clarkson Ave., Potsdam, NY 13699 (USA)

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  • The NSF is acknowledged for the support of this research (grant numbers CBET 0966526 and CBET 0966574).

Abstract

The enzymogel nanoparticle made of a magnetic core and polymer brush shell demonstrates a novel type of remote controlled phase-boundary biocatalysis that involves remotely directed binding to and engulfing insoluble substrates, high mobility, and stability of the catalytic centers. The mobile enzymes reside in the polymer brush scaffold and shuttle between the enzymogel interior and surface of the engulfed substrate in the bioconversion process. Biocatalytic activity of the mobile enzymes is preserved in the enzymogel while the brush-like architecture favors the efficient interfacial interaction when the enzymogel spreads over the substrate and extends substantially the reaction area as compared with rigid particles.

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