Noninvasive Measurement of the Mechanical Force Generated by Motor Protein EF-G during Ribosome Translocation

Authors

  • Dr. Li Yao,

    1. Department of Chemistry, University of Houston, Houston, TX 77204 (USA)
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  • Yue Li,

    1. Department of Biology and Biochemistry, University of Houston, Houston, TX 77204 (USA)
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  • Te-Wie Tsai,

    1. Department of Chemistry, University of Houston, Houston, TX 77204 (USA)
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  • Prof. Shoujun Xu,

    Corresponding author
    1. Department of Chemistry, University of Houston, Houston, TX 77204 (USA)
    • Shoujun Xu, Department of Chemistry, University of Houston, Houston, TX 77204 (USA)

      Yuhong Wang, Department of Biology and Biochemistry, University of Houston, Houston, TX 77204 (USA)

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  • Prof. Yuhong Wang

    Corresponding author
    1. Department of Biology and Biochemistry, University of Houston, Houston, TX 77204 (USA)
    • Shoujun Xu, Department of Chemistry, University of Houston, Houston, TX 77204 (USA)

      Yuhong Wang, Department of Biology and Biochemistry, University of Houston, Houston, TX 77204 (USA)

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  • Support from the US National Science Foundation (grant number ECCS-1028328, S.X.) and the Welch Foundation (grant number E-1721, Y.W.) is acknowledged. This work is also supported in part by the NHARP of Texas (grant number 003652-0010-2011, Y.W.), Texas Center of Superconductivity at University of Houston and a GEAR grant (S.X.).

Abstract

original image

Der Krafthub eines Motorproteins: Das Motorprotein EF-G erzeugt einen Krafthub von 89 pN während der Translokation eines Ribosoms (siehe Bild). Diese mechanische Kraft wird durch Messung der Kraft-induzierten Dissoziation einer Serie von DNA-mRNA-Doppelsträngen erhalten. Eine Abnahme des magnetischen Signals weist auf die Dissoziation hin.

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