These authors contributed equally to this work.
An Electroactive Biotin-Doped Polypyrrole Substrate That Immobilizes and Releases EpCAM-Positive Cancer Cells†
Article first published online: 20 MAR 2014
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 126, Issue 18, pages 4685–4690, April 25, 2014
How to Cite
Jeon, S., Moon, J.-M., Lee, E. S., Kim, Y. H. and Cho, Y. (2014), An Electroactive Biotin-Doped Polypyrrole Substrate That Immobilizes and Releases EpCAM-Positive Cancer Cells. Angew. Chem., 126: 4685–4690. doi: 10.1002/ange.201309998
This work was supported by a National Cancer Center grant from the Republic of Korea (1310180-1).
- Issue published online: 25 APR 2014
- Article first published online: 20 MAR 2014
- Manuscript Revised: 14 JAN 2014
- Manuscript Received: 18 NOV 2013
- Republic of Korea. Grant Number: 1310180-1
- Elektrische Reize;
- Leitfähige Polymere;
The specific capture and remotely controlled release of the EpCAM-positive cancer cells from biotin-doped polypyrrole (Ppy) films in response to an electrical potential is presented. As Ppy allows the direct incorporation of biotin molecules during the electrochemical process, densely packed biotin molecules can serve as the binding sites for streptavidin-tagged biomolecular complexes. This study demonstrates not only the enhanced capture and enrichment of EpCAM-positive cancer cells but also “on-demand” release of the viable cells from conductive Ppy in an electrical-potential-dependent way. This novel approach is of great importance in a diverse range of applications, and in particular in cancer diagnostics and screening.